Addgene

We narrowed to 4,045 results for: PRS

Showing: 451 - 480 of 4045 results

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pRS423-ProSgH

Plasmid

#163974

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BXG

Plasmid

#158145

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (w/ TAG codon)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-BYG

Plasmid

#158144

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment (wild-type)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-tglA

Plasmid

#134673

Purpose

Expresses His6-TglA in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglA

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETB BS2mT4L1

Plasmid

#141203

Purpose

Chimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Planchestainer et al Catalysts 2018, 8(1), 40

Insert

BS2m-T4L1

Use

Tags

Expression

Bacterial

Mutation

E11Q

Promoter

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-ECM_unstable

Plasmid

#127607

Purpose

high-copy URA-selectable plasmid, Mut ECM33 intron (unstable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Unstable ECM33 intron (Mut)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-ECM33_intron

Plasmid

#127596

Purpose

low-copy URA-selectable plasmid, ECM33 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-RPL27B_intron

Plasmid

#127604

Purpose

low-copy URA-selectable plasmid, RPL27B intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

RPL27B intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-APS3_intron

Plasmid

#127605

Purpose

low-copy URA-selectable plasmid, APS3 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

APS3 intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-HNT2_intron

Plasmid

#127603

Purpose

low-copy URA-selectable plasmid, HNT2 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

HNT2 intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(NT)

Plasmid

#138258

Purpose

Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS414-ATG41

Plasmid

#113080

Purpose

Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.

Depositor

Daniel Klionsky

Article

Yao et al Autophagy. 2015;11(12):2288-99. doi: 10.

Insert

ATG41

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

ATG41 endogenous promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-50

Plasmid

#111315

Purpose

pRS314 snu114-50

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-50 (C928R)

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETA-Prp28

Plasmid

#111285

Purpose

Prp28 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP28

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315 U2 IIc+

Plasmid

#111357

Purpose

U2 snRNA with IIc+ mutation on a pRS315 plasmid

Depositor

Christine Guthrie

Insert

snR20

Use

Tags

Expression

Yeast

Mutation

stem II IIc+ mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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sub2-5 pRS315

Plasmid

#111302

Purpose

sub2-5 in pRS315

Depositor

Christine Guthrie

Article

Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

Insert

SUB2

Use

Tags

Expression

Yeast

Mutation

sub2-5

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-15

Plasmid

#111312

Purpose

pRS314 snu114-15

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-15 (K146I)

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-14

Plasmid

#111311

Purpose

snu114-14

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-14

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 prp8-1

Plasmid

#111306

Purpose

prp8-1

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

prp8-1 ( G1801D )

Promoter

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-40

Plasmid

#111314

Purpose

snu114-40

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-40 (M842R)

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-30

Plasmid

#111313

Purpose

snu114-30

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-30 (G646R)

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-60

Plasmid

#111316

Purpose

pRS314 snu114-60

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-60 (K939stop)

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 HA-SNU114

Plasmid

#111318

Purpose

HA-SNU114

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pRSETB

Plasmid

#104500

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only
pRSF_ZmSCE1b-Myc

Plasmid

#91930

Purpose

Bacterial expression of maize SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

ZmSCE1b-Myc

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro3GFP

Plasmid

#82367

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro3GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/N149C/S202C

Promoter

T7

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETa-Amrose v2

Plasmid

#79538

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 2

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose v2

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.9

Plasmid

#78179

Purpose

Expresses-rsGreen0.9 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.9

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7b

Plasmid

#78178

Purpose

Expresses rsGreen0.7b in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7b

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa

Plasmid

#78183

Purpose

Expresses pcDronpa in E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

pcDronpa

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge