We narrowed to 22,191 results for: his

1. SJZ6-HIS5

   Plasmid

   #228259

   Purpose

   Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   his5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 S.c.

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET26b_AT118-H-V5-His

   Plasmid

   #222850

   Purpose

   E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-H
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCrhaB2_ApyD_ RBS_ApyAOHIS

   Plasmid

   #226247

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   ApyO
   ApyH
   ApyI
   ApyS
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pD(Ct)_3C-TS-His10

   Plasmid

   #223893

   Purpose

   4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3C-TwinStrep-His10
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pD(Nt)_His6-TEV

   Plasmid

   #223872

   Purpose

   4G-cloning donor for N-terminal His6-TEV tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-TEV
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pD(Nt)_His6-FLAG3

   Plasmid

   #223874

   Purpose

   4G-cloning donor for N-terminal His6-FLAG3 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-FLAG3
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pD(Nt)_His6-SUMO

   Plasmid

   #223879

   Purpose

   4G-cloning donor for N-terminal His6-SUMO tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-SUMO
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD(Ct)_3C-His8

   Plasmid

   #223887

   Purpose

   4G-cloning donor for C-terminal 3C-His8 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3C-His8
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pD(Ct)_CPD-His10

   Plasmid

   #223890

   Purpose

   4G-cloning donor for C-terminal CPD-His10 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   CPD-His10
   

   Use

   Golden-gate donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET26b_AT118-A-V5-His

    Plasmid

    #222849

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-A
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHis-WzzE

    Plasmid

    #196661

    Purpose

    Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

    Plasmid

    #220477

    Purpose

    heterologous expression of SSAT1 under endogenous speG E. coli promoter

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    SSAT1 (SAT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOPINVHH_A10-5_His

    Plasmid

    #201720

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH_A10-5 (S lama glama (llama))
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOPINVHH_B5-5_His

    Plasmid

    #201721

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH B5-5 (S lama glama (llama))
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ClhN-HIS5

    Plasmid

    #207014

    Purpose

    Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHyg-His6

    Plasmid

    #212776

    Purpose

    C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pet28b_His_RAD23A-UBA1

    Plasmid

    #201549

    Purpose

    Expresses UBA1 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 160-205 of DNA polymerase i…

    Promoter

    T7

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PATL2_G288-Y543_BV_his

    Plasmid

    #194760

    Depositor

    Cheryl Arrowsmith

    Insert

    PATL2 (PATL2 Human)
    

    Use

    Baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    July 19, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. OLFML3_123-406_AviN_HisC

    Plasmid

    #195871

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    OLFML3 (OLFML3 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMpGWBhis03-Citrine

    Plasmid

    #188557

    Purpose

    Binary vector for the expression of Citrine with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

    Depositor

    Yutaka Kodama

    Article

    Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

    Insert

    Citrine and MpIGPDm
    

    Use

    CRISPR; Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMC-6-NnHISPS-9

    Plasmid

    #197754

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    FBP: NnHISPS (luciferin synthesis)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. COL11A1_36-250_HisN_AviC

    Plasmid

    #195878

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    COL11A1 (COL11A1 Human)
    

    Use

    Pfhmsp-avic-lic-n, baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. COL11A1_1570-1806_HisN_AviC

    Plasmid

    #195879

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    COL11A1 (COL11A1 Human)
    

    Use

    Pfhmsp-avic-lic-n, baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. COL25A1_53-654_AviN_HisC

    Plasmid

    #195880

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    COL25A1 (COL25A1 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. NXPH1_22-271_AviN_HisC

    Plasmid

    #195872

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NXPH1 (NXPH1 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NTN1_39-453_AviN_HisC

    Plasmid

    #195873

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NTN1 (NTN1 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ECE1_101-770_HisN_AviC

    Plasmid

    #195875

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ECE1 (ECE1 Human)
    

    Use

    Pfhmsp-avic-lic-n, baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLIB_BSSHI_6xHisTEV-WIPI2d

    Plasmid

    #190863

    Purpose

    Plasmid for the expression of purification of WIPI2d: SMC Internal reference: SMC1216

    Depositor

    Sascha Martens

    Insert

    WIPI2 (WIPI2 Human)
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    SNP C to G at position 4562 (Val)

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His8OphMA-21mer.

    Plasmid

    #189670

    Purpose

    His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

    Depositor

    James Naismith, Markus Künzler

    Article

    Emmanuel et al bioRxiv 2022.07.21.500988

    Insert

    His8OphMATEV18mer
    

    Use

    Tags

    His8 tagged

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. His8OphMADeltaC6-18mer.

    Plasmid

    #189671

    Purpose

    His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

    Depositor

    James Naismith, Markus Künzler

    Article

    Emmanuel et al bioRxiv 2022.07.21.500988

    Insert

    His8OphMA∆C6-TEV18mer
    

    Use

    Tags

    His8 tagged

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pmPlum-histone H3

    Plasmid

    #179700

    Purpose

    visualization of nucleus/chromosome

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Mol Biol 2016, 5:2.

    Insert

    Histone H3 (H3c10 Mouse)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHIS8-3_DL121

    Plasmid

    #171953

    Purpose

    Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

    Depositor

    Kimberly Reynolds

    Article

    McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

    Insert

    Fusion of ecDHFR and asLOV2 at position 121 of DHFR
    

    Use

    Tags

    8-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a(+)-HisTim12_CtoA

    Plasmid

    #170281

    Purpose

    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

    Insert

    yeast Tim12 (TIM12 Budding Yeast)
    

    Use

    Tags

    TEV-cleavable N-terminal His tag (on backbone)

    Expression

    Bacterial

    Mutation

    mutated two non-conserved Cys to Ala

    Promoter

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET19b-HisDAD2

    Plasmid

    #165996

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    HisDAD2
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Expression

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKDsgRNA-hisF

    Plasmid

    #154922

    Purpose

    aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting hisF
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHis-Hgd

    Plasmid

    #163091

    Purpose

    Expressing mouse Hgd in bacterial cells

    Depositor

    Joseph Takahashi

    Article

    Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

    Insert

    Hgd (Hgd Mouse)
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHis-Laforin

    Plasmid

    #157989

    Purpose

    Expresses Laforin in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Choi et al J Kor Chem Soc, 2015; 59(5): 466-470.

    Insert

    Laforin (EPM2A Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFA6a-S6-FLAG:HIS3MX6

    Plasmid

    #134439

    Purpose

    PCR-based C-terminal S6-FLAG tag

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    S6-FLAG
    

    Use

    Pcr-based tagging in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge