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pRK5-FLAG-SZT2 2241-end

Plasmid

#87039

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AA 2241-end

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SZT2 1-1021

Plasmid

#87036

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AA 1-1021

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Halo RagB

Plasmid

#112753

Purpose

express Halo-RagB

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Halo-RagB (RRAGB Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SZT2 2241-end

Plasmid

#87040

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

AA 2241-end

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC2-MTHFD1-3xFLAG

Plasmid

#217429

Purpose

Lentiviral overexpression of human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

MTHFD1 (MTHFD1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pKH160_FLAG-APEX2-YIPF4 LIR mut

Plasmid

#214979

Purpose

Flag-APEX2 tagged LIR mutant YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2_YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH145_mCherry-YIPF4 LIR mut

Plasmid

#214973

Purpose

mCherry tagged LIR mutant YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mCherry-YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_BC-pyr_comp_sig_pep

Plasmid

#202132

Purpose

Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Pyrenoid compartment signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Sept. 6, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-ER_sig_pep

Plasmid

#202136

Purpose

Level 0 plasmid containing a kozak consensus sequence and a ER signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Endoplasmic reticulum signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-mito_sig_pep

Plasmid

#202134

Purpose

Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Mitochondria signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-sec_sig_pep

Plasmid

#202133

Purpose

Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Secretion signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-chl_sig_pep

Plasmid

#202131

Purpose

Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Chloroplast signal peptide

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL2-1 ConjEpi PtEF2bsd Pt49202tHMGRVenusThrFLAG

Plasmid

#139964

Purpose

Over-expression of N-terminal truncated version of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_tHMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAWH-IRP

Plasmid

#133896

Purpose

Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

IRP

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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HA-mus-Bnip3_deltaExon3

Plasmid

#100793

Purpose

Mammalian expression of Bnip3_deltaExon3 splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mouse-Bnip3_deltaExon3 (Bnip3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Exon3 deletion

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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FAS promoter luciferase

Plasmid

#8890

Depositor

Bruce Spiegelman

Article

Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

Insert

Fatty Acid Synthetase Promoter (Fasn Rat, unknown - rat or mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pKH115_GALNT2-keima

Plasmid

#214969

Purpose

Golgiphagy flux reporter GALNT2-keima

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GALNT2-keima (GALNT2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Rheb-N153T

Plasmid

#19997

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 - N153T (RHEB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed Asparagine 153 to Threonine

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pHTNW

Plasmid

#136403

Purpose

Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p27-IRES-mCherry

Plasmid

#80143

Purpose

retroviral expression of p27 and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

p27 (CDKN1B Human)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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