We narrowed to 17,336 results for: URE

1. pVeBtA2

   Plasmid

   #238987

   Purpose

   Mammalian expression of bovine arrestin-2 long isoform with Venus N-terminal fusion

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

   Insert

   arrestin-2 long (ARRB2 Bovine)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVeBtA2-3A

   Plasmid

   #238991

   Purpose

   Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

   Insert

   arrestin-2 long (ARRB2 Bovine)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   I386A,V387A,F388A

   Promoter

   CMV

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BtA3CL

   Plasmid

   #238992

   Purpose

   In vitro transcription of mutant bovine arrestin-3 short isoform

   Depositor

   Vsevolod Gurevich

   Article

   Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

   Insert

   arrestin-3 short (ARR3 Bovine)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   C17S, C60 V, C126S, C141L, C151V, C243V, C252V, C…

   Promoter

   SP6

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVEA3-delta7

   Plasmid

   #234802

   Purpose

   Mammalian expression of bovine arrestin-3 (deleted residues 176, 177, 178, 182-185)

   Depositor

   Vsevolod Gurevich

   Article

   Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

   Insert

   Arrestin-3 (ARR3 Bovine)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   deleted residues 176, 177, 178, 182-185

   Promoter

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p3VEA3-3A

   Plasmid

   #234797

   Purpose

   Mammalian expression of bovine arrestin-3 (I385A,V386A,F387A)

   Depositor

   Vsevolod Gurevich

   Article

   Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

   Insert

   Arrestin-3 (ARR3 Bovine)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   I385A,V386A,F387A

   Promoter

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ReaLiSM A1-26link-GPIba+2cys

   Plasmid

   #59580

   Purpose

   express VWF A1-GPIba ReaLiSM construct in mammalian cells

   Depositor

   Timothy Springer

   Article

   Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

   Insert

   VWF A1-linker-GPIba (VWF Human)
   

   Use

   Tags

   6His

   Expression

   Mammalian

   Mutation

   Promoter

   chick beta-actin

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

7. P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

   Plasmid

   #239972

   Purpose

   Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

   Plasmid

   #239974

   Purpose

   PiggyBac plasmid for inducible PEmax expression and Puromycin selection

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

   Plasmid

   #183790

   Purpose

   A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Inserts

   P2A-eGFP-TargetBC-5xTAPE-1
   pegRNA-InsertBC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   G>A in the gRNA scaffold- please see depositor…

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Rlucrarr3-Flash5

    Plasmid

    #74133

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Rlucrarr3-Flash6

    Plasmid

    #74134

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Rlucrarr3-Flash1

    Plasmid

    #74129

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. Rlucrarr3-Flash4

    Plasmid

    #74132

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

14. Rlucrarr3-Flash3

    Plasmid

    #74131

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

15. Rlucrarr3-Flash2

    Plasmid

    #74130

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pRMC2-sec:msfGFP

    Plasmid

    #194914

    Purpose

    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Human a4 nicotinic subunit crystallization construct

    Plasmid

    #85841

    Purpose

    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

    Insert

    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Human b2 nicotinic subunit crystallization construct

    Plasmid

    #85842

    Purpose

    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

    Insert

    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    C-terminal StrepII tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRMC2-tat:msfGFP

    Plasmid

    #194915

    Purpose

    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCX4-cMyc

    Plasmid

    #36119

    Depositor

    Steven Dowdy

    Article

    Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only