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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,334 results for: fas

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Showing: 301 - 320 of 2334 results

  1. pFastBac1/MBP-His

    Plasmid
    #38008
    Depositor
    Michael J Fox Foundation MJFF
    Insert
    MBP (Maltose binding protein)
    Use
    Baculovirus expression
    Tags
    6xHIS
    Expression
    Insect
    Promoter
    Polyhedrin
    Available since
    Oct. 1, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pFastBacMam4_N_Flag_His8_eGFP_TEV

    Plasmid
    #178808
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    Flag, His8, eGFP, TEV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178808-plasmid-map-sequence-id-351925

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  6. SPLICSFAST-LY-MT-P2A

    Plasmid
    #214897
    Purpose
    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites
    Depositor
    Tito Calì
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPLICSFAST-LY-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214897-plasmid-map-sequence-id-423483

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  7. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Publication
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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  11. pFastbac1 Nterminal GST

    Plasmid
    #128058
    Purpose
    Baculovirus expression vector with N terminal GST
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Tags
    GST
    Expression
    Insect
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128058-plasmid-map-sequence-id-255102

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  12. SPLICSFAST-ER-MT-P2A

    Plasmid
    #214892
    Purpose
    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPLICSFAST-ER-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214892-plasmid-map-sequence-id-423478

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  13. ER-SPLICSs-cFAST10

    Plasmid
    #210431
    Purpose
    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    ER-SPLICSs-cFAST10
    Tags
    Flag
    Expression
    Mammalian
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210431-plasmid-map-sequence-id-415626

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  15. mEos2-Fascin-C-10

    Plasmid
    #57376
    Purpose
    Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Publication
    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fascin (FSCN1 Human)
    Tags
    mEos2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

    Plasmid
    #179459
    Purpose
    Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)
    Depositor
    Francois St-Pierre
    Publication
    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00
    Has service
    AAV1
    Insert
    JEDI-2P-Kv
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179459-plasmid-map-sequence-id-353727

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  20. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

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Showing: 301 - 320 of 2334 results