Addgene

Showing: 301 - 320 of 6224 results

Results per page:

Sort results by:

pSB819-PKR-hum-LYA

Plasmid

#20050

Depositor

Harmit Malik

Article

Elde et al Nature. 2008 Nov 30. ():.

Insert

Protein kinase R (EIF2AK2 Human)

Use

Tags

Expression

Yeast

Mutation

F489Y, S492A

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
Flag-M4-AR-K630A-K632A

Plasmid

#171263

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys630Ala-Lys632Ala (AR-K630A-K632A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Lys630Ala-Lys632Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Lys630Ala-Lys632Ala (AR-K630A-K632A)

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_CDK6_SFTA3

Plasmid

#205791

Purpose

Express mEGFP-tagged fusion protein, CDK6_SFTA3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CDK6_SFTA3 (CDK6, SFTA3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPET-PKR/PPase

Plasmid

#42934

Depositor

James Cole

Article

Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

Inserts

protein kinase R (EIF2AK2 Human)
phage lambda phosphatase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG-hAR

Plasmid

#89080

Purpose

mammalian expression of FLAG-tagged human androgen receptor

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

hAR (AR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
pBSc + MShal

Plasmid

#16166

Depositor

Larry Salkoff

Article

Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

Insert

Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
GST-AR-624-919

Plasmid

#104200

Purpose

Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

Insert

human androgen receptor aa 624-919 (AR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRR-AR-5Z

Plasmid

#23058

Depositor

Charles Miller

Article

Miller et al Plasmid. 2009 Dec 3. ():.

Insert

AR (AR Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
Cdk6-DN-HA

Plasmid

#1869

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pHis-zfEpoa

Plasmid

#65611

Purpose

Express soluble His-tagged zebrafish erythropoietin in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

Insert

erythropoietin (epoa Zebrafish)

Use

Tags

(His)6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIREN-RetroQ-CDK6-shRNA

Plasmid

#25789

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK6 (CDK6 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
Cdk6-DN

Plasmid

#1867

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
GST-AR-4-52

Plasmid

#104199

Purpose

Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

human androgen receptor aa 4-52 (AR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR-W741C-S81A-P82A

Plasmid

#171250

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82…

Promoter

CMV

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR-W741C-S81A-P82G

Plasmid

#171251

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82…

Promoter

CMV

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

Plasmid

#141452

Purpose

B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

Cdk6 (Cdk6 Mouse)

Use

Mouse Targeting; Dre / rox

Tags

C-terminal fusion of T2A-TagRFPT-dre-PBD*

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-AR-1-36

Plasmid

#104192

Purpose

Expresses human AR aa 1-36 in pGEX-5x-1 bact expression vector with GST

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-36 (AR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3HA-AR-LBD

Plasmid

#89111

Purpose

mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 1999 Dec 24;274(52):37219-2

Insert

hAR ligand binding domain (AR Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

contains Ligand Binding Domain (624-919)

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex-1 AC30_eGFP

Plasmid

#82715

Purpose

Expresses a GFP-tagged recombinant adenylyl cyclase.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)

Use

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge