We narrowed to 23,940 results for: nes

1. NES-EGFP-PH-ARNO2G-I303Ex2

   Plasmid

   #116868

   Purpose

   PIP3 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   CYTH2 (CYTH2 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amind acids 252-399, isoleucine 303 changed to gl…

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-MMLVgag-3xNES-ABE8e-NG

   Plasmid

   #181754

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCas13-inactive M3M14nes

   Plasmid

   #157855

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0056 - LwCas13a-msfGFP-NES

   Plasmid

   #105815

   Purpose

   Expresses active LwaCas13a-NES for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NES and msfGFP-NES-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-MMLVgag-3xNES-ABE7.10-NG

   Plasmid

   #181753

   Purpose

   MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE7.10-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-CMV-NES-jRGECO1a

   Plasmid

   #61563

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRGECO1a
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Use

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-NES-jRCaMP1a

   Plasmid

   #61562

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1a
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-GFP-TurboID-NES_pLX304

    Plasmid

    #202019

    Purpose

    expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNESx2-EGFP-C1-PABDx2-Spo20

    Plasmid

    #220082

    Purpose

    PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    Spo20 (SPO20 Budding Yeast)
    

    Use

    Tags

    X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

    Expression

    Mammalian

    Mutation

    amino acids 51-91, tandem dimer

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX304-Flag-APEX2-NES

    Plasmid

    #92158

    Purpose

    Cytosolic APEX2 construct

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    Flag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSIIbsr-Eevee-ROCK-NES

    Plasmid

    #209918

    Purpose

    A FRET biosensor for ROCK activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    Eevee-ROCK-NES
    

    Use

    Lentiviral

    Tags

    ECFP and YPet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

    Plasmid

    #194681

    Purpose

    CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-CMV-NES-jRCaMP1b

    Plasmid

    #63136

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRCaMP1b
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

    Plasmid

    #194683

    Purpose

    CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

    Plasmid

    #194686

    Purpose

    CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

    Plasmid

    #194685

    Purpose

    CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194687

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-CMV-NES-fRCaMP2

    Plasmid

    #125250

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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