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pHR_PGK_SNIPR_Notch3 del-NRR

Plasmid

#188355

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-del-NRR-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch3-NRRdeletion-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 2, V319A

Plasmid

#188366

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_V319A, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_V319A_Notch1-JMD_Gal4VP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VP16

Plasmid

#183927

Purpose

Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-STAT2

Plasmid

#183931

Purpose

Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-STAT2 (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-Rta

Plasmid

#183930

Purpose

Optogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-Rta (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VPR

Plasmid

#183928

Purpose

Optogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VPR (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-FUSN

Plasmid

#183932

Purpose

Optogenetic PHR domain coupled to GFP and FUSN domain with a high phase separation propensity; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-FUSN (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-nE-mHP1alpha

Plasmid

#181890

Purpose

Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-nE-mHP1alpha (Cbx5 Mouse, Mustard Weed)

Use

Tags

PHR-mCherry

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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IBAR-Cry2PHR-WH2

Plasmid

#176605

Purpose

Cry2PHR with N-terminal IBAR domain and C-terminal WH2 domain

Depositor

Robert Hughes

Article

Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

Insert

IBAR-Cry2PHR-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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Cry2PHR-IBAR-WH2

Plasmid

#176603

Purpose

Cry2PHR with C-terminal IBAR and WH2 domains

Depositor

Robert Hughes

Article

Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

Insert

Cry2PHR-mCherry-IBAR-WH2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hHP1β

Plasmid

#103829

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hHP1β (CRY2, CBX1 Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5mut

Plasmid

#103828

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5mut (CRY2, KAT2A Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-hGCN5

Plasmid

#103826

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hGCN5 (CRY2, KAT2A Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-H2B-mGFP

Plasmid

#173892

Purpose

for H2B labeling

Depositor

Ron Vale

Article

Yan et al J Cell Biol. 2021 Feb 1;220(2). pii: 211

Insert

H2B (H2BC21 Human)

Use

Lentiviral

Tags

mGFP

Expression

Mutation

Promoter

SFFV

Available since

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_HA-BirA-ER

Plasmid

#113897

Purpose

Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-mCh-Cry2WT

Plasmid

#101223

Purpose

FUS(1-214) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag9-iCAM-1

Plasmid

#205209

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG9

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag9 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag17-iCAM-1

Plasmid

#205204

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG17

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag17 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-CRY2-NLS

Plasmid

#145259

Purpose

Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CTLA-4-mGFP

Plasmid

#223594

Purpose

Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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