We narrowed to 704 results for: transfection

1. HA-mios in pRK5

   Plasmid

   #154212

   Purpose

   N-terminal HA-tagged Mios cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   Mios
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   cDNA sequence has been codon optimized using huma…

   Promoter

   CMV

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-GLUT5 in pRK5

   Plasmid

   #154205

   Purpose

   N-terminal HA-tagged GLUT5 cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   SLC2A5 (SLC2A5 Human)
   

   Use

   Tags

   N terminal HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. shh10

   Plasmid

   #64867

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

   Insert

   shh10 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   I319V, N451D, D532N

   Promoter

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAS_4xMx PylT_GFP 150TAG

   Plasmid

   #140016

   Purpose

   Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG, K46E (Please see depositor comments below)

   Promoter

   EF1

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHcRed-NPM1wt-C1

   Plasmid

   #131818

   Purpose

   Expresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAS_4xMma PylT sfGPF 150TAG

   Plasmid

   #154766

   Purpose

   plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, U25C in PylT

   Promoter

   EF1

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC-AdEvolve-DEST

   Plasmid

   #131692

   Purpose

   A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDOE11 Lat52:mCherry transient

   Plasmid

   #124249

   Purpose

   Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

   Depositor

   Attila Fehér

   Article

   Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MPRA Empty Vector

   Plasmid

   #204865

   Purpose

   Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

   Depositor

   Philipp Maass

   Article

   Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL4.23-Fosprom

    Plasmid

    #188113

    Purpose

    Expresses luciferase under control of Fos promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

    Insert

    Fos promoter (Fos Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Fos endogenous promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SEH1L in pRK5

    Plasmid

    #154216

    Purpose

    Untagged SEH1L cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    SEH1L (SEH1L Human)
    

    Use

    Tags

    NONE

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-DDK

    Plasmid

    #137764

    Purpose

    Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GFP
    

    Use

    Tags

    DDK

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOO2-GW

    Plasmid

    #135097

    Purpose

    Gateway vector for expression of proteins in Xenopus oocytes

    Depositor

    Wolf Frommer

    Article

    Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

    Type

    Empty backbone

    Use

    Bacterial expression vector for generating sp6 tr…

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTarget TMEM97-P2A-mCardinal

    Plasmid

    #157747

    Purpose

    Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

    Depositor

    Andrew Kruse

    Article

    Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

    Insert

    TMEM97 (TMEM97 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. BCL9-BirA*

    Plasmid

    #109147

    Purpose

    To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    BCL9 (BCL9 Human)
    

    Use

    Tags

    BirA(R118G)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAS_4xMx PylT C41CA_GFP 150TAG

    Plasmid

    #140017

    Purpose

    Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIhh742bp-Luc

    Plasmid

    #53605

    Purpose

    Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pAS_4xhyb PylT sfGFP 150TAG

    Plasmid

    #154771

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIhh1.3K-Luc

    Plasmid

    #53604

    Purpose

    Reporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pDOE11 mas:mTq2 transient

    Plasmid

    #124248

    Purpose

    Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

    Depositor

    Attila Fehér

    Article

    Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge