We narrowed to 866 results for: transfection
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Plasmid#118655PurposeTransient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway DonorDepositorInsertSMN2-DN-RG6-SA
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-715MRPprom-MRPcucuc
Plasmid#87199PurposeExpression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiencyDepositorAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H GC editing reporter
Plasmid#198884PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralExpressionMammalianMutationY93H, Gly92(GGT-to-GGG)PromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT sfGFP 150TAG
Plasmid#154767Purposeplasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAGPromoterEF1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPWCh
Plasmid#124238PurposeGateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.DepositorTypeEmpty backboneExpressionInsectPromoterUASpAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCX:Gachapin-C2-P2A-mCherry-NLS
Plasmid#251137PurposeGachapin-C with a transfection marker mCherry-NLSDepositorInsertGachapin-C2
TagsP2A-mCherry-NLSExpressionMammalianPromoterChicken β-actin promoterAvailable SinceFeb. 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
Multi-Janus-Spacer-1x_pKG1126
Plasmid#246375PurposeMulti-Janus spacer for Galloway Lab Golden Gate cloning scheme. Used as a "filler" plasmid in transfection experiments.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
TVMV-HRV-3C Proteases-mCherry
Plasmid#243796PurposeEncodes for mammalian expression of TVMV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.DepositorInsertTVMV and HRV-3C proteases
ExpressionMammalianAvailable SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
TVMV-TEV Proteases-mCherry
Plasmid#243797PurposeEncodes for mammalian expression of TVMV and TEV protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.DepositorInsertTVMV and TEV proteases
ExpressionMammalianAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-hGpr75
Plasmid#236746PurposeExpress 3xFlag N-terminal tagged wild-type human GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mGL-bGH
Plasmid#235299PurposemGreenLantern expression to serve as a transfection marker, EF1a-drivenDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-mGL-bGH
Plasmid#235255PurposemGreenLantern expression to serve as a transfection marker, EFS-drivenDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianPromoterEFS (human EF1a short, intronless)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-mGpr75
Plasmid#236744PurposeExpress 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-mGpr75-L144P
Plasmid#236745PurposeExpress 3xFlag N-terminal tagged mouse GPR75-L144P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-tagBFP-bGH
Plasmid#235257PurposetagBFP expression to serve as a "filler" plasmid for transfectionsDepositorInserttagBFP
UseSynthetic BiologyExpressionMammalianMutationSilent mutations to remove BsaI sites in tagBFPPromoterEF1a (human EF1a)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_GFP
Plasmid#235611PurposeEncodes GFP. For use in transfections as a negative control without epitope tags.DepositorInsertGFP
ExpressionMammalianAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCBE-dGFP
Plasmid#226861PurposeConstitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by Y93H mutation, that can be reactivated by active cytosine base editingDepositorInsertEGFP
ExpressionMammalianMutationY93HPromoterCMVAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-del72-136-pEGFP-C3
Plasmid#191411PurposeTransfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).DepositorInsertBcl-2-interacting killer (BIK Human)
TagsVenusExpressionMammalianMutationwith deletion of amino acids 72-136 (del72-136) i…PromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-GgVCL/*TBM
Plasmid#162790PurposeMUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cellsDepositorArticleInsertMutated Gallus gallus vinculin (G454V) (VCL Chicken)
TagsIRES-GFPExpressionBacterial and MammalianMutationchanged Gly 454 to ValPromoterbeta actinAvailable SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_GFP 150TAG
Plasmid#140016PurposePlasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG, K46E (Please see depositor comments below)PromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only