We narrowed to 13,238 results for: BASE

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AID-C12*-n'Cas9-BE4max

Plasmid

#216730

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-n'Cas9-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265C

Plasmid

#164715

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible haid-cbe_v01 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265E1

Plasmid

#164718

Purpose

Gateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible a3a-cbe_v01 entry clone

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265E3

Plasmid

#164720

Purpose

Gateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

eA3A-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible ea3a-cbe_v01 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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hPGK-ABE7.10-P2A-GFP

Plasmid

#162511

Purpose

Express ABE7.10-P2A-GFP by hPGK promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

hPGK

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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EFS-ABE7.10-P2A-GFP

Plasmid

#162510

Purpose

Express ABE7.10-P2A-GFP by EFS promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-202

Plasmid

#135344

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-202

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pC0096 RESCUE truncation (Cas13 delta 892-1095)

Plasmid

#130663

Purpose

CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b(delta 892-1095)-ADAR2dd

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-NLuc-MBP-6xHis

Plasmid

#135931

Purpose

Expresses NanoLuciferase in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)

Plasmid

#114212

Purpose

Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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CaYin1

Plasmid

#33067

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1

Tags

6 His tag

Expression

Bacterial

Promoter

ARA promoter

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBacT7-S1T3D

Plasmid

#33282

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S1 (S1 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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p667-UBC-SCYL1-V5-miniTurbo_IDG-K

Plasmid

#189911

Purpose

Gateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

SCYL1 (SCYL1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-MARK1_IDG-K

Plasmid

#170582

Purpose

Gateway destination clone of MARK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-MARK1 (MARK1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK15_IDG-K

Plasmid

#158179

Purpose

Gateway destination clone of CDK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK15 (CDK15 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p667-UBC-PKMYT1-V5-miniTurbo_IDG-K

Plasmid

#135246

Purpose

Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PKMYT1-V5-miniTurbo (PKMYT1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-GSK3B_IDG-K

Plasmid

#158166

Purpose

Gateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-GSK3B (GSK3B Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-TurboID-V5-BRSK2_IDG-K

Plasmid

#135272

Purpose

Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TurboID-V5-BRSK2 (BRSK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-Site2

Plasmid

#24513

Depositor

Kazuo Kinoshita

Article

Uemura et al PLoS One. 2010 . 5(3):e9846.

Insert

Split RFP-GFP chimera

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

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