We narrowed to 9,591 results for: EPO

1. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. H2B-GFP-Aurora B WT

   Plasmid

   #184042

   Purpose

   Expresses H2B-GFP fused to Aurora B WT

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   AURKB (AURKB Human)
   

   Use

   Tags

   EGFP and H2B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

   Plasmid

   #184396

   Purpose

   Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

   Plasmid

   #128164

   Purpose

   UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).

   Depositor

   Marcus Noyes

   Article

   Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

   Insert

   10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
   

   Use

   Synthetic Biology

   Tags

   FLAG and zif268

   Expression

   Bacterial

   Mutation

   Promoter

   UV5

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR Human CASP9 -2

   Plasmid

   #198420

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Mutation

   Promoter

   U6; CMV

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR Human CASP9 -1

   Plasmid

   #198419

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Mutation

   Promoter

   U6; CMV

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)

   Plasmid

   #128165

   Purpose

   UV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).

   Depositor

   Marcus Noyes

   Article

   Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

   Insert

   10 amino acid glycine/serine linker + 5alanines
   

   Use

   Synthetic Biology

   Tags

   FLAG and zif268

   Expression

   Bacterial

   Mutation

   Promoter

   UV5

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SOX17-NLS-tdTomato-EPG

   Plasmid

   #210466

   Purpose

   Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJOP-HTT-HR18Q

    Plasmid

    #87228

    Purpose

    HTT HR arms with 18 CAG repeats and piggyBac selection cassette

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a driving selection cassette

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-miRFP-6xFLAG

    Plasmid

    #161614

    Purpose

    Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    miRFP-6xFLAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human synapsin 1 promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-mRuby3-6xFLAG

    Plasmid

    #161613

    Purpose

    Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    mRuby3-6xFLAG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human synapsin 1 promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TetO-FUW-Hes1

    Plasmid

    #61534

    Purpose

    Expresses Hes1 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Hes1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-mTagBFP2-FRB-Rab7

    Plasmid

    #214273

    Purpose

    Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-DMD-Donor

    Plasmid

    #60605

    Purpose

    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    Hygromycin resistant gene
    

    Use

    Knock-in donor vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV[Exp]-Puro-EF-1α>{H2B-RFP}

    Plasmid

    #201121

    Purpose

    RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. KAT6B-Tol2

    Plasmid

    #113384

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pUC57-4eCOL2A1

    Plasmid

    #97211

    Purpose

    For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

    Depositor

    Ryan Porter

    Article

    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

    Insert

    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    

    Use

    Vector is designed for cloning and generation of …

    Tags

    Expression

    Mutation

    Promoter

    COL2A1 regulatory elements

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

    Plasmid

    #154775

    Purpose

    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAA in GFP reporter, UUA anticodon and G10A, …

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pegRNA-HEK3_del1-5-NGG

    Plasmid

    #185478

    Purpose

    pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    HEK3_del1-5-NGG pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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