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Showing: 8001 - 8050 of 167150 results

  1. 857 pBabe puroL PTEN G129R

    Plasmid
    #10772
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    G129R
    Available Since
    Sept. 27, 2005
    Availability
    Academic Institutions and Nonprofits only

  3. L4018

    Plasmid
    #1614
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2204, SV40NLS, SV40NLS, SV40NLS, SV40NLS,…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1614-plasmid-map-sequence-id-224

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  4. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Expression
    Bacterial
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

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  5. tdEos-Alpha1-Catenin-C-18

    Plasmid
    #57574
    Purpose
    Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Alpha1-Catenin
    Tags
    tdEos
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. R777-E195 Hs.PRKAB1

    Plasmid
    #70479
    Purpose
    Gateway ORF clone of human PRKAB1 [NM_006253.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PRKAB1 (PRKAB1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70479-plasmid-map-sequence-id-125928

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  7. mTurquoise-PLK1-N-16

    Plasmid
    #55575
    Purpose
    Localization: Centrosomes, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    PLK1 (PLK1 Human)
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa

    Plasmid
    #86451
    Purpose
    Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)
    Depositor
    Gregor Bucher
    Insert
    Hsp_p-GAL4Delta-SV40;attp
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86451-plasmid-map-sequence-id-171105

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  10. pEBG-hOSR1-Flag-D164A

    Plasmid
    #185363
    Purpose
    For mammalian expression of human GST-OSR1-Flag-D164A (kinase-dead)
    Depositor
    Ayaz Najafov
    Insert
    OXSR1 (OXSR1 Human)
    Tags
    Flag and GST
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185363-plasmid-map-sequence-id-363589

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  12. pCWXPG-UBI-PDX1:VP16

    Plasmid
    #114305
    Purpose
    Constitutive expression of a PDX1:VP16 fusion protein
    Depositor
    Patrick Salmon
    Insert
    PDX1-VP16 (Pdx1 Mouse)
    Use
    Lentiviral
    Available Since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    114305-plasmid-map-sequence-id-218746

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  14. mCherry-T2A-LSS-SGFP2

    Plasmid
    #112944
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Insert
    mCherry and LSS-SGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112944-plasmid-map-sequence-id-220241

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  15. R777-E149 Hs.PAK2

    Plasmid
    #70433
    Purpose
    Gateway ORF clone of human PAK2 [NM_002577.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PAK2 (PAK2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70433-plasmid-map-sequence-id-125882

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  16. pET28a SUMO Nup53xl 162-267

    Plasmid
    #86002
    Purpose
    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53
    Depositor
    Wolfram Antonin
    Type
    Empty backbone
    Tags
    SUMO
    Expression
    Bacterial
    Available Since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    86002-plasmid-map-sequence-id-171435

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  17. CDKL5A-c014

    Plasmid
    #53686
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CDKL5A
    Use
    Baculovirus expression
    Tags
    His6-TEV
    Mutation
    Activating mutations T169D / Y171E
    Promoter
    polyhedrin promoter
    Available Since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. T16m

    Plasmid
    #121035
    Purpose
    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P55 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121035-plasmid-map-sequence-id-245903

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  19. mEos4b-MKS1-N-18

    Plasmid
    #62776
    Purpose
    Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Michael Davidson, Loren Looger
    Insert
    MKS1
    Tags
    mEos4b
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

    Plasmid
    #84388
    Purpose
    Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mTagBFP2
    Expression
    Mammalian
    Promoter
    CMV immediate early
    Available Since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84388-plasmid-map-sequence-id-169692

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  22. mFGF3

    Plasmid
    #22086
    Depositor
    Gail Martin
    Insert
    FGF3 (Fgf3 Mouse)
    Use
    Bacterial
    Available Since
    Sept. 17, 2009
    Availability
    Academic Institutions and Nonprofits only

  23. PP-CDPK1 (3Q5I)

    Plasmid
    #28280
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-CDPK1 (PB000158.03.0 Plasmodium berghei)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. mTurquoise-PMP-N-10

    Plasmid
    #55576
    Purpose
    Localization: Peroxisomal Membrane, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    PMP (PXMP2 Human)
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. R777-E215 Hs.RALA

    Plasmid
    #70499
    Purpose
    Gateway ORF clone of human RALA [NM_005402.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RALA (RALA Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70499-plasmid-map-sequence-id-125948

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  26. SnFR_S062

    Plasmid
    #182810
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    S062-CholineSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182810-plasmid-map-sequence-id-358310

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  27. mEmerald-Myotilin-C-14

    Plasmid
    #54196
    Purpose
    Localization: Cytoskeleton, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Myotilin (MYOT Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. pAS2-his-24::mNeptune

    Plasmid
    #124332
    Purpose
    Expressing Nuclear Localized mNeptune
    Depositor
    Andrew Leifer
    Insert
    mNeptune (his-24 Nematode)
    Expression
    Worm
    Available Since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124332-plasmid-map-sequence-id-241916

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  30. HOXB5-pECE

    Plasmid
    #20985
    Depositor
    Corey Largman
    Insert
    HOXB5 (HOXB5 Human)
    Expression
    Mammalian
    Available Since
    Aug. 14, 2009
    Availability
    Academic Institutions and Nonprofits only

  31. R777-E154 Hs.PAK4-nostop

    Plasmid
    #70438
    Purpose
    Gateway ORF clone of human PAK4 [NM_005884.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PAK4 (PAK4 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70438-plasmid-map-sequence-id-125887

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  33. pAS2-his-24::tagBFP

    Plasmid
    #124320
    Purpose
    Expressing Nuclear Localized tagBFP
    Depositor
    Andrew Leifer
    Insert
    tagBFP (his-24 Nematode)
    Expression
    Worm
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124320-plasmid-map-sequence-id-241687

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  34. pAW8-aid-eDSR-Turg1:kanMX6

    Plasmid
    #169362
    Purpose
    C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169362-plasmid-map-sequence-id-333207

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  35. AK5A-c001

    Plasmid
    #53674
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    AK5A
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    R156M, Bacterial expression for structure determi…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. L4666

    Plasmid
    #1668
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, pat3MbLS, gfpN, IVSR, g…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1668-plasmid-map-sequence-id-278

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  37. pIC215

    Plasmid
    #69736
    Purpose
    Mammalian Expression of PELP1
    Depositor
    Iain Cheeseman
    Insert
    PELP1 (PELP1 Human)
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    E927G compared to GenBank reference sequence NP_0…
    Available Since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  38. pIC214

    Plasmid
    #69735
    Purpose
    Mammalian Expression of Tousled-like kinase 1 (TLK1)
    Depositor
    Iain Cheeseman
    Insert
    Tousled-like kinase 1 (TLK1) (TLK1 Human)
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Available Since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. mKO2-ZO1-C-14

    Plasmid
    #57906
    Purpose
    Localization: Tight Junctions, Excitation: 551, Emission: 565
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Tags
    mKO2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    57906-plasmid-map-sequence-id-210571

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  40. SRS-MTBD(19/19), AB(monomer), ux

    Plasmid
    #22389
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Tags
    His and Myc
    Expression
    Bacterial
    Available Since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22389-plasmid-map-sequence-id-10296

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  41. pET15b-At 14-3-3 omicron

    Plasmid
    #44926
    Depositor
    Robert Ferl
    Insert
    14-3-3 omicron (GRF11 Mustard Weed)
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 14, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. pAS2-his-24::tagRFP

    Plasmid
    #124330
    Purpose
    Expressing Nuclear Localized tagRFP
    Depositor
    Andrew Leifer
    Insert
    tagRFP (his-24 Nematode)
    Expression
    Worm
    Available Since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124330-plasmid-map-sequence-id-241912

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  44. Dendra2-Cx43-7

    Plasmid
    #57713
    Purpose
    Localization: Gap Junctions, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    Cx43
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. ZP57 Lef1

    Plasmid
    #16885
    Depositor
    Randall Moon
    Insert
    lef1 (lef1 Zebrafish)
    Expression
    Bacterial
    Available Since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only

  46. XE170 mouse Wnt-1-myc-pcDNA3

    Plasmid
    #16854
    Depositor
    Randall Moon
    Insert
    Wnt1 (Wnt1 Mouse)
    Use
    Xenopus expression
    Tags
    myc (internal)
    Expression
    Mammalian
    Available Since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only

  48. R777-E269 Hs.RASSF9

    Plasmid
    #70553
    Purpose
    Gateway ORF clone of human RASSF9 [NM_005447.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF9 (RASSF9 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70553-plasmid-map-sequence-id-126002

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  49. pET15b-Fab1 (S. aureus)

    Plasmid
    #61691
    Purpose
    Inducible expression of the FabI enzyme from S. aureus
    Depositor
    Michael Johnson
    Insert
    FabI
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. mApple-Paxillin-KM-N-18

    Plasmid
    #54936
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Paxillin-KM
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 8001 - 8050 of 167150 results