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pAAV-syn-SnFR-gamma2-minWPRE

Plasmid

#165497

Purpose

AAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisation

Depositor

Andrew Plested

Article

Hao et al Elife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)

Use

AAV

Promoter

human Synapsin

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37087

Purpose

Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-syn-axon-jGCaMP8m-WPRE

Plasmid

#186042

Purpose

jGCaMP8m expression in axons under the control of the synapsin promoter

Depositor

Loren Looger

Insert

axon-jGCaMP8m

Use

AAV

Tags

GAP43-6xHis

Expression

Mammalian

Promoter

synapsin

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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57_pAAV-ProA14-CatCh-GFP-WPRE

Plasmid

#125897

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA14

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP682-P2A-GFP

Plasmid

#197153

Purpose

Expresses the protein of iRFP682-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP682-P2A-GFP

Use

AAV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PdTK-CAG-mCh-[uBglII]

Plasmid

#107280

Purpose

puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-synaptoPAC-minWPRE

Plasmid

#153100

Purpose

Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Chronos-mT-Sapphire-NRN

Plasmid

#149692

Purpose

For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

Plasmid

#122102

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-loxP-GRAB_DA2m-loxP

Plasmid

#189751

Purpose

Excludes sensor expression in Cre-positive neurons

Depositor

Yulong Li

Article

Dai et al Cell Rep. 2022 Aug 23;40(8):111246. doi:

Insert

Dopamine sensor GRAB_DA2m

Use

AAV

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-Flex-dTomato-Fishell_7

Plasmid

#83894

Purpose

Cre recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

tdTomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/5'USS-GFP

Plasmid

#197884

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

Plasmid

#128587

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato (codon diversified version)

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

EF1α (1.1 kb short version)

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1 KanR foundation lox casette

Plasmid

#132388

Purpose

Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

G418 resistance

Use

CRISPR and Cre/Lox

Expression

Mammalian

Promoter

promoterless

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-TagRFP658-P2A-GFP

Plasmid

#178971

Purpose

Expresses TagRFP658 and GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

TagRFP658-P2A-GFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-GFP-shRNA-scramble

Plasmid

#182503

Purpose

Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

hSyn promoter

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange-NRN

Plasmid

#149693

Purpose

For packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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124_pAAV-ProC3-CatCh-GFP-WPRE

Plasmid

#125939

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC3

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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p87AAVEGFP hp47phox (S303/304/328D)

Plasmid

#112250

Purpose

mutant p47phox

Depositor

Simone Di Giovanni

Insert

p47phox (NCF1 Human)

Use

AAV

Tags

EGFP

Mutation

S303/304/328D

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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