Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 9,405 results for: Coli

Showing: 4831 - 4860 of 9405 results

  1. pAIP4_5MDT

    Plasmid
    #234186
    Purpose
    Heterologous protein expression of rpb7-binding protein seb1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5MDT (seb1 Fission Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234186-plasmid-map-sequence-id-464151

    Enlarge

  2. pAIP4_2GAS

    Plasmid
    #234145
    Purpose
    Heterologous protein expression of isoflavone reductase (IFR) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2GAS
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234145-plasmid-map-sequence-id-464111

    Enlarge

  3. MZB205

    Plasmid
    #228490
    Purpose
    Empty 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    10xHis-SUMO(Smt3)-Avitag-HRV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228490-plasmid-map-sequence-id-455387

    Enlarge

  4. pBP-Tag_phoA

    Plasmid
    #204107
    Purpose
    Type 0 tag part
    Depositor
    Mikhail Shapiro
    Insert
    Signal peptide from E. coli PhoA protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    204107-plasmid-map-sequence-id-437314

    Enlarge

  5. pBP-Tag_dsbA

    Plasmid
    #204106
    Purpose
    Type 0 tag part
    Depositor
    Mikhail Shapiro
    Insert
    Signal peptide from E. coli DsbA protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204106-plasmid-map-sequence-id-418230

    Enlarge

  6. C99m

    Plasmid
    #121003
    Purpose
    MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121003-plasmid-map-sequence-id-236141

    Enlarge

  7. pExp-Sumo

    Plasmid
    #112574
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112574-plasmid-map-sequence-id-217243

    Enlarge

  8. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

    Enlarge

  9. pET32a-LIC

    Plasmid
    #62310
    Purpose
    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis, S-tag, and Trx-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62310-plasmid-map-sequence-id-326570

    Enlarge

  10. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112568-plasmid-map-sequence-id-217224

    Enlarge

  11. pExp-NusA

    Plasmid
    #112571
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112571-plasmid-map-sequence-id-220666

    Enlarge

  12. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

    Enlarge

  13. pExp-GST

    Plasmid
    #112566
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-GST and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112566-plasmid-map-sequence-id-217229

    Enlarge

  14. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P43
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

    Enlarge

  16. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

    Enlarge

  17. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

    Enlarge

  18. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112558-plasmid-map-sequence-id-217235

    Enlarge

  19. pOPINEneo-3C-GFP

    Plasmid
    #53534
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Use
    Tags
    GFP-His8
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53534-plasmid-map-sequence-id-83411

    Enlarge

  20. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63722-plasmid-map-sequence-id-106345

    Enlarge

  21. Sc FLAG-S6-rad24-pRSFDuet

    Plasmid
    #240734
    Purpose
    Over-express FLAG-S6-rad24 in E. coli
    Depositor
    Michael O'Donnell
    Insert
    FLAG-S6-rad24 (RAD24 Budding Yeast)
    Use
    Tags
    3X FLAG followed by S6
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240734-plasmid-map-sequence-id-482405

    Enlarge

  22. Sc [ddc1 + rad17]-pET

    Plasmid
    #240740
    Purpose
    Over-express Sc ddc1 & Sc rad17 in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    ddc1 (DDC1 Budding Yeast)
    rad17 (RAD17 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240740-plasmid-map-sequence-id-482365

    Enlarge

  23. Sc Rad27-S6- pET24

    Plasmid
    #239205
    Purpose
    Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    Rad27 (RAD27 Budding Yeast)
    Use
    Tags
    S6
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239205-plasmid-map-sequence-id-474608

    Enlarge

  24. Sc FLAG-RFC1-pRSFDuet

    Plasmid
    #239201
    Purpose
    Overexpress Sc RFC1 (N-terminal 3X FLAG tag) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    RFC1 (RFC1 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239201-plasmid-map-sequence-id-474602

    Enlarge

  25. pAIP4_1YW5

    Plasmid
    #234235
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1YW5 (ESS1 Candida albicans)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234235-plasmid-map-sequence-id-464199

    Enlarge

  26. pAIP4_4HS5

    Plasmid
    #234223
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4HS5
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234223-plasmid-map-sequence-id-464188

    Enlarge

  27. pAIP4_1UKF

    Plasmid
    #234214
    Purpose
    Heterologous protein expression of cysteine protease avirulence protein AvrPphB in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UKF
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234214-plasmid-map-sequence-id-464179

    Enlarge

  28. pAIP4_1LU4

    Plasmid
    #234207
    Purpose
    Heterologous protein expression of soluble secreted antigen MPT53 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1LU4 (mpt53 Mycobacterium tuberculosis)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234207-plasmid-map-sequence-id-464172

    Enlarge

  29. pAIP4_1KAF

    Plasmid
    #234209
    Purpose
    Heterologous protein expression of middle transcription regulatory protein motA in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1KAF (motA Tequatrovirus T4)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234209-plasmid-map-sequence-id-464174

    Enlarge

  30. pAIP4_1TP9

    Plasmid
    #234200
    Purpose
    Heterologous protein expression of peroxiredoxin-2 (Prx) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TP9 (LOC7476296 Populus trichocarpa)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234200-plasmid-map-sequence-id-464165

    Enlarge

Showing: 4831 - 4860 of 9405 results