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pHWBF09exp

Plasmid

#130833

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP1delTM

Plasmid

#130841

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of transmembrane domain

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKG1delKASH

Plasmid

#130842

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP2del4ec

Plasmid

#130843

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Use

Unspecified

Tags

eGFP-FLAG-HA

Mutation

Deletion of terminal four amino acid residues

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKG1delKASHec

Plasmid

#130846

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of KASH domain

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHWBF07EC

Plasmid

#130828

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHWBF09EC

Plasmid

#130829

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
ECPK1D

Plasmid

#130830

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHWBF07exp

Plasmid

#130832

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMB2a-TelR

Plasmid

#204985

Purpose

Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMB2b-TelR

Plasmid

#204986

Purpose

Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB2-A-TelR

Plasmid

#204987

Purpose

Level 2 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB3-A-TelR

Plasmid

#204991

Purpose

Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0019

Plasmid

#205992

Purpose

pNpuDnaE(C)_HygR(53-341)_3HA

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)-HygR

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTHY_P0019_T2c_BCD_0.1K

Plasmid

#200057

Purpose

Bicistronic RBS 0.1K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.1K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0028_T2c_BCD_18K

Plasmid

#200048

Purpose

Bicistronic RBS 18K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 18K

Use

Synthetic Biology

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0021_T2c_BCD_0.45K

Plasmid

#200055

Purpose

Bicistronic RBS 0.45K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.45K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0020_T2c_BCD_0.2K

Plasmid

#200056

Purpose

Bicistronic RBS 0.2K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.2K

Expression

Bacterial

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0026_T2c_BCD_8.5K

Plasmid

#200050

Purpose

Bicistronic RBS 8.5K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 8.5K

Use

Synthetic Biology

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE1176

Plasmid

#202774

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0023_T2c_BCD_1.5K

Plasmid

#200053

Purpose

Bicistronic RBS 1.5K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 1.5K

Expression

Bacterial

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0027_T2c_BCD_13K

Plasmid

#200049

Purpose

Bicistronic RBS 13K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 13K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0025_T2c_BCD_4.6K

Plasmid

#200051

Purpose

Bicistronic RBS 4.6K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 4.6K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0024_T2c_BCD_2.3K

Plasmid

#200052

Purpose

Bicistronic RBS 2.3K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 2.3K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0022_T2c_BCD_0.75K

Plasmid

#200054

Purpose

Bicistronic RBS 0.75K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 0.75K

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0030_T2c_BCD_25K

Plasmid

#200045

Purpose

Bicistronic RBS 25K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 25K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHY_P0029_T2c_BCD_23K

Plasmid

#200047

Purpose

Bicistronic RBS 23K Part

Depositor

Ross Thyer

Article

Jansen et al ACS Synth Biol. 2023 Jun 16;12(6):1608-1

Insert

BCD 23K

Use

Synthetic Biology

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc

Plasmid

#199759

Purpose

Elongation reporter construct to quantify elongation duration of 4_CGA stalling

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-4xArg[CGA]-nLuc

Expression

Yeast

Promoter

TetO7

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Plasmid

#199760

Purpose

Elongation reporter construct to quantify elongation duration of 6_CGA stalling

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

Plasmid

#199761

Purpose

Elongation reporter construct to quantify elongation duration of 6_AGA stalling

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-6xArg[AGA]-cLuc

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB2880

Plasmid

#193117

Purpose

A2 Proximal promoter sequence consisting of the target sequences for the gRNA1 (GB1838) and gRNA2 (GB1839) flanked by random sequences.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A2) G1aG2b.6

Use

Synthetic Biology

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEF-S15-FRB-Bla1

Plasmid

#190916

Purpose

Protein fragment complementation

Depositor

Jakob Reiser

Article

Ou et al Gene Ther. 2013 Jan;20(1):43-50. doi: 10

Insert

S15-FRB-Bla1 fusion protein

Tags

N-terminal fragment of BLA fused to c-Src and FRB…

Expression

Mammalian

Promoter

CEF

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRC6-ProC

Plasmid

#198430

Purpose

mkate2 fluorescent reporter gene under control of promoter ProC. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProC and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRC6-ProD

Plasmid

#198431

Purpose

mkate2 fluorescent reporter gene under control of promoter ProD. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProD and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-RLuc-HsAsb9-3UTR_X

Plasmid

#147949

Purpose

Mammalian Expression of HsAsb9-3UTR

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsAsb9-3UTR (ASB9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNanos1-del1-39_X

Plasmid

#147932

Purpose

Mammalian Expression of HsNanos1-del1-39

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-del1-39 (NANOS1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNanos1-del1-58_X

Plasmid

#147933

Purpose

Mammalian Expression of HsNanos1-del1-58

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-del1-58 (NANOS1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNanos1-del40-56_X

Plasmid

#147934

Purpose

Mammalian Expression of HsNanos1-del40-56

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-del40-56 (NANOS1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-CeSmg5_S

Plasmid

#147456

Purpose

Mammalian Expression of CeSmg5

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5 (smg-5 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-CeSmg5-G91E_S

Plasmid

#147457

Purpose

Mammalian Expression of CeSmg5-G91E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5-G91E (smg-5 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-CeSmg5-Q94E_S

Plasmid

#147459

Purpose

Mammalian Expression of CeSmg5-Q94E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg5-Q94E (smg-5 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-CeSmg7_S

Plasmid

#147465

Purpose

Mammalian Expression of CeSmg7

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7 (smg-7 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-CeSmg7-G80E_S

Plasmid

#147466

Purpose

Mammalian Expression of CeSmg7-G80E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7-G80E (smg-7 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-CeSmg7-T83E_S

Plasmid

#147467

Purpose

Mammalian Expression of CeSmg7-T83E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7-T83E (smg-7 Nematode)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-CeSMG5_1-420_S

Plasmid

#147468

Purpose

Bacterial Expression of CeSMG5_1-420

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSMG5_1-420 (smg-5 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-CeSMG5_1-420-G91E_S

Plasmid

#147469

Purpose

Bacterial Expression of CeSMG5_1-420-G91E

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSMG5_1-420-G91E (smg-5 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuetMP-CeSmg7_1-395_S

Plasmid

#147475

Purpose

Bacterial Expression of CeSmg7_1-395

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

CeSmg7_1-395 (smg-7 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsSmg5-Q123E_T

Plasmid

#147555

Purpose

Mammalian Expression of HsSmg5-Q123E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-Q123E (SMG5 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1-dsRNAres_N

Plasmid

#147029

Purpose

Insect Expression of DmNot1-dsRNAres

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmNot1-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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