We narrowed to 5,762 results for: Chia

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pCMV5D HA-Δ10 PPM1H

Plasmid

#207335

Purpose

Mammalian expression of HA-Δ10 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ10

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ20 PPM1H

Plasmid

#207336

Purpose

Mammalian expression of HA-Δ20 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ20

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ34 PPM1H

Plasmid

#207337

Purpose

Mammalian expression of HA-Δ34 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ34

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37 PPM1H

Plasmid

#207338

Purpose

Mammalian expression of HA-Δ37 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ44 PPM1H

Plasmid

#207339

Purpose

Mammalian expression of HA-Δ44 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ44

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ71 PPM1H

Plasmid

#207340

Purpose

Mammalian expression of HA-Δ71 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ71

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-hNRG1a

Plasmid

#199235

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

Insert

Neuregulin (NRG1 Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mCsnk2b - 2

Plasmid

#198506

Purpose

lentiviral stable expression of mCsnk2b gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mCsnk2b (Csnk2b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R3tdTomatoi2

Plasmid

#185969

Purpose

Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick

Use

Synthetic Biology

Promoter

PR

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 integrase (GB1561)

Plasmid

#160586

Purpose

Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)

Plasmid

#155136

Purpose

Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

GST and HA

Expression

Bacterial

Mutation

D243G/L244G/F245G/M247G/S248G/M249G/A250G

Promoter

tac

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-H93R

Plasmid

#140739

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation H93R and GFP as two separate proteins

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-T2A-PTENH93R (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

H93R

Promoter

h

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-PtenN276S

Plasmid

#140737

Purpose

Lentiviral vector to overexpress the PTEN mutation N276S as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

PTEN – phosphatase and tensin homolog (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

N276S

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-PtenW274L

Plasmid

#140736

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation W274L as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-PTEN (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

W274L

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-PtenD252G

Plasmid

#140735

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation D252G as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-PTEN (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D252G

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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FUG-PtenH93R

Plasmid

#140733

Purpose

Lentiviral vector to overexpress the autism-associated PTEN mutation H93R as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-PTEN (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

H93R

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBB0238-TEV-His12

Plasmid

#137038

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66635.2

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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Human INPP5A

Plasmid

#129587

Purpose

Expression of human INPP5A in bacteria

Depositor

Christophe Erneux

Article

Erneux et al Eur J Biochem. 1995 Dec 1;234(2):598-602

Insert

Type I inositol 5-phosphatase (INPP5A Human)

Tags

Maltose binding protein

Expression

Bacterial

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-S18

Plasmid

#127826

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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