We narrowed to 6,087 results for: nls

1. Ezh2[FL]-dCas9

   Plasmid

   #100086

   Purpose

   Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   14056 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[FL]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-AR Q48

   Plasmid

   #86428

   Purpose

   Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   Androgen receptor (AR) (AR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Poly-glutamine expansion, G130D

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1-AR Q0

   Plasmid

   #86427

   Purpose

   Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   androgen receptor (AR) (AR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the poly-glutamine expansion

   Promoter

   CMV

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

   Plasmid

   #188774

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-FUS_LC_WT

   Plasmid

   #98653

   Purpose

   Expresses MBP-tagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CMVc-Cas9

   Plasmid

   #106431

   Purpose

   Expresses SpCas9 from the CMVc promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMVc

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBP-FUS_FL_12E

   Plasmid

   #98652

   Purpose

   Expresses MBP-tagged full length phosphomimetic FUS

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   12 S/T to E

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. G9A[SET]-dCas9

   Plasmid

   #100089

   Purpose

   Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   G9A (EHMT2 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-G9A[SET]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   G9A[SET] includes aa 829-1209

   Promoter

   CMV

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-MDFIC

   Plasmid

   #207835

   Purpose

   Tagged MDFIC for western blotting and imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   MDFIC (MDFIC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ucp1-Cre-miR122

    Plasmid

    #228408

    Purpose

    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Inserts

    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Ucp1 enhancer-promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MDFI

    Plasmid

    #207836

    Purpose

    Tagged MDFI for western blotting and imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    MDFI (MDFI Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CARPID dCasRx-BASU

    Plasmid

    #153303

    Purpose

    express CARPID dCasRx-BASU fusion protein in mammalian cells

    Depositor

    Liang Zhang, Jian Yan

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    dCasRx-BASU
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188773

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-FUS_FL_6E

    Plasmid

    #98655

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    6 S/T to E

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-nEF-dCas9

    Plasmid

    #106430

    Purpose

    Expresses SpdCas9 from the nEF promoter in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    nEF-dCas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. shRNA Caspase 1 (pSicoR mCherry)

    Plasmid

    #53575

    Purpose

    shRNA against human pro-caspase 1

    Depositor

    Warner Greene

    Article

    Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

    Insert

    shRNA Caspase 1 (CASP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; EF1a for mCherry

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pEvol-pAcFRS.1.t1

    Plasmid

    #73545

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.1.t1
    pAcFRS.1.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. OA-1136J

    Plasmid

    #153023

    Purpose

    Express His-MBP-TEV-CasRx recombinant protein

    Depositor

    Omar Akbari

    Article

    Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

    Insert

    CasRx
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-PEmax NG

    Plasmid

    #187894

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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