We narrowed to 5,882 results for: pcDNA 3.1

1. Opto-D2R

   Plasmid

   #106068

   Purpose

   Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-D2R
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Opto-GPR139

   Plasmid

   #106044

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR139; Opto-GPR139; D10)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR139
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. human-GFAP-EGFP

   Plasmid

   #169473

   Purpose

   We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   EGFP-hGFAP (GFAP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YFP Gamma 8

   Plasmid

   #36106

   Depositor

   Narasimhan Gautam

   Article

   Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

   Insert

   G protein subunit Gamma 8 (GNG8 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2012

   Availability

   Academic Institutions and Nonprofits only

5. Opto-M2R

   Plasmid

   #106070

   Purpose

   Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-M2R
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV-SpyCas9

   Plasmid

   #113033

   Purpose

   SpyCas9 expression in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SpyCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-CBS (WT)

   Plasmid

   #182923

   Purpose

   Mammalian expression plasmid for human CBS

   Depositor

   Philip Eaton

   Article

   Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

   Insert

   Cystathionine Beta-Synthase (CBS Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Opto-M3R

   Plasmid

   #106071

   Purpose

   Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M3R; Opto-M3R; H1; 724)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-M3R
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SPLICS Mt-ER- Po Short P2A

   Plasmid

   #164118

   Purpose

   Detect the short Peroxisomes-mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP Mt-ER- Po Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pY026

    Plasmid

    #84741

    Purpose

    Expresses huAsCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huAsCpf1
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. VinTS-tCRMod-GGSGGS9

    Plasmid

    #111765

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSL2783 (pTnsC-VP64_Vch)

    Plasmid

    #200896

    Purpose

    Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsC-VP64
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAL175

    Plasmid

    #22276

    Purpose

    Expresses YFP-PIF for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHLuc Control

    Plasmid

    #141091

    Purpose

    pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mH2A1.2-CT-GFP

    Plasmid

    #45169

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Halo-NEMO

    Plasmid

    #187899

    Purpose

    Mammalian cell expression of Halo-tagged NEMO

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    NEMO (Ikbkg Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. perbB2-ECFP

    Plasmid

    #40268

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    erbB2 (ERBB2 Human)
    

    Use

    Tags

    ecfp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG SMG1 D2335A/N2340K

    Plasmid

    #199575

    Purpose

    Expresses FLAG-tagged kinase dead SMG1

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D2335A and N2340K

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VinTS-tCRMod-GGSGGS7

    Plasmid

    #111764

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSYN-PC5-003

    Plasmid

    #42501

    Depositor

    Robert Peters

    Article

    Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

    Insert

    PC5 (PCSK5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FLAG HDAC5 WT

    Plasmid

    #32213

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

23. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. YFP Gamma 2

    Plasmid

    #36102

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 2 (GNG2 Bovine)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pTS1051-Tier1-PhCMV-4xCox8

    Plasmid

    #169490

    Purpose

    Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p4B5-EGFP

    Plasmid

    #140324

    Purpose

    Detection of Dengue virus infection in live cells

    Depositor

    Alan Rothman

    Article

    Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

    Insert

    DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    2 conservative n.t. differences near 5' end …

    Promoter

    CMV

    Available since

    May 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSL2669 (pTnsABf_Vch)

    Plasmid

    #200897

    Purpose

    Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsA-TnsB
    

    Use

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pY027

    Plasmid

    #84742

    Purpose

    Expresses huLbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huLbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CREB3L3

    Plasmid

    #99509

    Purpose

    to express CREB3L3 in mammalian system

    Depositor

    Dong-Yan Jin

    Article

    Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

    Insert

    CREB3L3 (Creb3l3 Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mcherry Beta 1

    Plasmid

    #36111

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein beta subunit B1 (GNB1 Bovine)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only