Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,549 results for: pET

Showing: 8101 - 8150 of 15549 results

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RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Tags

SNAP

Expression

Bacterial and Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R)-9myc

Plasmid

#212787

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-yeGFP

Plasmid

#212768

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-yeGFP

Plasmid

#212766

Purpose

C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R)-yeGFP

Plasmid

#212767

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo-yeGFP

Plasmid

#212771

Purpose

C-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo(K48R)-yeGFP

Plasmid

#212772

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo(K63R)-yeGFP

Plasmid

#212773

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-His6

Plasmid

#212776

Purpose

C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-His6

Plasmid

#212777

Purpose

C-terminal PCR-based tagging with CUbo-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R)-His6

Plasmid

#212778

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-His6

Plasmid

#212779

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-yeGFP-His6

Plasmid

#212782

Purpose

C-terminal PCR-based tagging with CUbo-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-9myc

Plasmid

#212786

Purpose

C-terminal PCR-based tagging with CUbo-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-9myc

Plasmid

#212788

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo-AID*-GFP

Plasmid

#212770

Purpose

C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo(K63R)

Plasmid

#212764

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo(K48R)

Plasmid

#212763

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo

Plasmid

#212762

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-His7-NUbo

Plasmid

#212761

Purpose

N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-NUbo

Plasmid

#212760

Purpose

N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKE011

Plasmid

#207912

Purpose

Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS018

Plasmid

#207911

Purpose

Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS017

Plasmid

#207909

Purpose

Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS020

Plasmid

#207915

Purpose

Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKE012

Plasmid

#207914

Purpose

Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKE010

Plasmid

#207910

Purpose

Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS019

Plasmid

#207913

Purpose

Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvH-CtPAN3_448-555_Y

Plasmid

#148093

Purpose

Bacterial Expression of CtPAN3_448-555

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

CtPAN3_448-555

Expression

Bacterial

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmGIGYF_331-374-GST-V5His6_AH

Plasmid

#148934

Purpose

Insect Expression of DmGIGYF_331-374

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_331-374 (Gyf Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-HA-DmGIGYF-dsRNAres_AH

Plasmid

#148928

Purpose

Insect Expression of DmGIGYF1-dsRNAres

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-dsRNAres (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-Dm4EHP_AH

Plasmid

#148930

Purpose

Insect Expression of Dm4EHP

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

Dm4EHP (4EHP Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-ScEap1p_101-122-GB1_AG

Plasmid

#148811

Purpose

Bacterial Expression of ScEap1p_101-122

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_101-122 (EAP1 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-ScEap1p_107-122-Y107IA108KS110T-GB1_AG

Plasmid

#148813

Purpose

Bacterial Expression of ScEap1p_107-122-Y107IA108KS110T

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_107-122-Y107IA108KS110T (EAP1 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-ScEap1p_123-150-GB1_AG

Plasmid

#148814

Purpose

Bacterial Expression of ScEap1p_123-150

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_123-150 (EAP1 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-SceIF4G_448-500-GB1_AF

Plasmid

#148716

Purpose

Bacterial Expression of SceIF4G_448-500

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4G_448-500 (TIF4631 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-Scp20_1-17-GB1_AF

Plasmid

#148717

Purpose

Bacterial Expression of Scp20_1-17

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

Scp20_1-17 (CAF20 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-Scp20_1-49_AF

Plasmid

#148718

Purpose

Bacterial Expression of Scp20_1-49

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

Scp20_1-49 (CAF20 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-Scp20_1-49-GB1_AF

Plasmid

#148719

Purpose

Bacterial Expression of Scp20_1-49

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

Scp20_1-49 (CAF20 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-CvH-CteIF4E_35-250-del183-202_AF

Plasmid

#148721

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-CvH-CteIF4E_40-250-del183-202_AF

Plasmid

#148723

Purpose

Bacterial Expression of CteIF4E_40-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_40-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpH-CteIF4E_35-250-del183-202_AF

Plasmid

#148725

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpH-SceIF4E_25-213_AF

Plasmid

#148726

Purpose

Bacterial Expression of SceIF4E_25-213

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4E_25-213 (CDC33 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpH-SceIF4E_25-213-I77AI81A_AF

Plasmid

#148727

Purpose

Bacterial Expression of SceIF4E_25-213-I77AI81A

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4E_25-213-I77AI81A (CDC33 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpH-SceIF4E_25-213-K42AK168AK187A_AF

Plasmid

#148728

Purpose

Bacterial Expression of SceIF4E_25-213-K42AK168AK187A

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4E_25-213-K42AK168AK187A (CDC33 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpH-SceIF4E_25-213-KKKKtoAAAA_AF

Plasmid

#148729

Purpose

Bacterial Expression of SceIF4E_25-213-KKKKtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4E_25-213-KKKKtoAAAA (CDC33 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpH-SceIF4E_35-213_AF

Plasmid

#148730

Purpose

Bacterial Expression of SceIF4E_35-213

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

SceIF4E_35-213 (CDC33 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-CteIF4G_996-1033-YLLtoAAA-GB1_AF

Plasmid

#148706

Purpose

Bacterial Expression of CteIF4G_966-1033-YLLtoAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4G_966-1033-YLLtoAAA

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-ScEap1p_80-122_AF

Plasmid

#148707

Purpose

Bacterial Expression of ScEap1p_80-122

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_80-122 (EAP1 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-ScEap1p_80-150-GB1_AF

Plasmid

#148708

Purpose

Bacterial Expression of ScEap1p_80-150

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_80-150 (EAP1 Budding Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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