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pHAGE-PDGFRB

Plasmid

#116770

Purpose

Lentiviral expression of PDGFRB

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRB (PDGFRB Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225703

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

Plasmid

#162380

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-VAChT [N425/45]

Plasmid

#190326

Purpose

Mammalian Expression Plasmid of anti-VAChT (Rat). Derived from hybridoma N425/45.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAChT (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-B2M

Plasmid

#116715

Purpose

Lentiviral expression of B2M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

B2M (B2M Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RET

Plasmid

#116787

Purpose

Lentiviral expression of RET

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RET (RET Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN

Plasmid

#116780

Purpose

Lentiviral expression of PTEN

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-TARPGamma2/4/8 [N245/36R]

Plasmid

#177522

Purpose

Mammalian Expression Plasmid of anti-TARPGamma2/4/8 (Rat). Derived from hybridoma N245/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody (Tarp Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE

Plasmid

#162382

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-S-tag [N56/9R]

Plasmid

#206600

Purpose

Mammalian Expression Plasmid of anti-S-tag (Bovine). Derived from hybridoma N56/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-S-tag (Bos taurus) recombinant Mouse monoclonal antibody (RNASE1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/8R]

Plasmid

#177572

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFBR2

Plasmid

#116800

Purpose

Lentiviral expression of TGFBR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

Plasmid

#228401

Purpose

Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

SREBP1 (SREBF1 Human)

Tags

mNeonGreen2x

Expression

Mammalian

Mutation

deleted N terminal activation domain (amino acids…

Promoter

EF1a

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pQhsCDtRNA-

Plasmid

#86015

Purpose

Multi-gene expression system with human box C/D proteins with U14

Depositor

Hong Li

Article

Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

Inserts

U14 (SNORD14A Human)
15.5K (SNU13 Human)
fibrillarin (FBL Human)
NOP56 (NOP56 Human)
NOP58 (NOP58 Human)

Use

Co-expression; purification

Tags

6x-His

Expression

Bacterial

Mutation

amino acids 1-401, amino acids 1-411, and amino a…

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

Plasmid

#225699

Purpose

Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K36M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Mutation

K36M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-VDAC1 [N152B/23R-2b]

Plasmid

#222171

Purpose

Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody. (VDAC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC1 [1F1R]

Plasmid

#177437

Purpose

Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Neuroligin-2 scFv [L107/95]

Plasmid

#206764

Purpose

Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/95 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/34R]

Plasmid

#220419

Purpose

Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225702

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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