We narrowed to 12,311 results for: BASE

1. pLR-NN

   Plasmid

   #31017

   Depositor

   Daniel Voytas, Adam Bogdanove

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   last NN repeat
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-VP6SA11

   Plasmid

   #89166

   Purpose

   Express Rotavirus SA11 VP6 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-3xFLAG-Puro

   Plasmid

   #207728

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLV hUbC-dCas9-T2A-GFP

   Plasmid

   #53191

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-VP7SA11

   Plasmid

   #89167

   Purpose

   Express Rotavirus SA11 VP7 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP7
   

   Use

   Tags

   *see below

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-TurboID-V5-CDK13_IDG-K

   Plasmid

   #135267

   Purpose

   Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TurboID-V5-CDK13 (CDK13 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Level 1 P4 TaU6 guide acceptor

   Plasmid

   #165600

   Purpose

   GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Level 1 P3 TaU6 guide acceptor

   Plasmid

   #165599

   Purpose

   GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCT-tRNA

   Plasmid

   #133813

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBJ2

    Plasmid

    #167131

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

    Plasmid

    #134662

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 5 (VAMP5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-His3MX6

    Plasmid

    #41596

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. TALED_Left-ND1-AD-E1347A

    Plasmid

    #183894

    Purpose

    Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-BE1

    Plasmid

    #73018

    Purpose

    Expresses BE1 with N-terminal His6 tag in E. Coli

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Spacer Switch Plasmid

    Plasmid

    #215346

    Purpose

    Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    OpaD spacer; Chloramphenicol Resistance Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_426

    Plasmid

    #179097

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dimer cleaved

    Plasmid

    #198199

    Purpose

    FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mVenus-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K both

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. TALED_Left-ND1-1397C-AD

    Plasmid

    #183892

    Purpose

    Expresses TALED_Left-ND1-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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