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Addgene

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Showing: 3241 - 3260 of 21642 results

  1. pLPC-dnMAML1-mVenus

    Plasmid
    #171023
    Purpose
    Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    MAML1 Dominant Negative (MAML1 Human)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    amino acids 12-74 only, hence conferring dominant…
    Promoter
    CMV
    Available since
    April 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    171023-plasmid-map-sequence-id-336789

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  6. pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217769
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400
    Promoter
    EF-1alpha promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217769-plasmid-map-sequence-id-435786

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  7. pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry

    Plasmid
    #172637
    Purpose
    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    2xFLAG-2xSTREP
    Expression
    Mammalian
    Mutation
    Pro287Ala
    Promoter
    CMV
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172637-plasmid-map-sequence-id-340160

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  10. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid
    #155189
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP
    Depositor
    Xiaoping Bao
    Article
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Insert
    beta-catenin (Ctnnb1 Mouse)
    Use
    Unspecified; Piggybac transposon
    Tags
    P2A eGFP NLS
    Expression
    Mammalian
    Mutation
    Sites phosphorylated by GSK-3beta have been mutat…
    Promoter
    TRE3G
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155189-plasmid-map-sequence-id-307963

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  13. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid
    #217768
    Purpose
    Fluorescent reporter for ceramide (for stable mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    EF-1alpha promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217768-plasmid-map-sequence-id-435794

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  14. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217767
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217767-plasmid-map-sequence-id-435788

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  15. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid
    #217766
    Purpose
    Fluorescent reporter for ceramide (for bacterial expression and purification)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217766-plasmid-map-sequence-id-435787

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Showing: 3241 - 3260 of 21642 results