We narrowed to 148,861 results for: ins;

1. p21(dPCNA)-mCerulean-NeoR

   Plasmid

   #215082

   Purpose

   Expressesp21(dPCNA)-mCerulean in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p21(dPCNA) denotes mutations M147A, D149A, F150A.

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p21(RxL)-mCerulean-NeoR

   Plasmid

   #215081

   Purpose

   Expresses p21(RxL)-mCerulean in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p21(RxL)-mCerulean-PuroR

   Plasmid

   #215078

   Purpose

   Expresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p21(dPCNA)-mCerulean-PuroR

   Plasmid

   #215079

   Purpose

   Expresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   p21(dPCNA) denotes mutations M147A, D149A, F150A.

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

   Plasmid

   #114074

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
   

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   S542R, K548V and N552R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABEpuro-ERBB2 C299S

   Plasmid

   #40988

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   C299S

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

   Plasmid

   #107943

   Purpose

   Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
   

   Tags

   3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

   Plasmid

   #92139

   Purpose

   Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS314-Cef1-M175I

   Plasmid

   #234118

   Purpose

   Suppressor screen mutant in Cef2

   Depositor

   Aaron Hoskins

   Insert

   Cef1
   

   Expression

   Yeast

   Mutation

   M175I

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS314-Cef1-Q193P

    Plasmid

    #234117

    Purpose

    Suppressor screen mutant in Cef1

    Depositor

    Aaron Hoskins

    Insert

    Cef1
    

    Expression

    Yeast

    Mutation

    Q193P

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS314-Cef1-A37V

    Plasmid

    #234119

    Purpose

    Suppressor screen mutant in Cef3

    Depositor

    Aaron Hoskins

    Insert

    Cef1
    

    Expression

    Yeast

    Mutation

    A37V

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET283xFlagPP7CPHis

    Plasmid

    #28174

    Depositor

    Kathleen Collins

    Insert

    PP7 coat protein optimized
    

    Tags

    6xhistidine and triple FLAG peptide

    Expression

    Bacterial

    Mutation

    C68A,C73A

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV500 A-ATF2

    Plasmid

    #33362

    Depositor

    Charles Vinson

    Insert

    ATF
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see comments)

    Promoter

    CMV

    Available Since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1C-RLuc-A90-HhR_O

    Plasmid

    #147073

    Purpose

    Insect Expression of A90-HhR

    Depositor

    Elisa Izaurralde

    Insert

    A90-HhR
    

    Expression

    Insect

    Mutation

    A127 instead of A90 (see comments below)

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS416-Syf1

    Plasmid

    #234113

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS416 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS414-Syf1

    Plasmid

    #234114

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS414 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 2PH-PLCdelta-GFP

    Plasmid

    #35142

    Depositor

    Sergio Grinstein

    Insert

    2PH-PLCdelta
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains 2 tandem PH-PLCdelta domains

    Promoter

    CMV

    Available Since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS424 Prp8 S1584F

    Plasmid

    #234122

    Purpose

    Suppressor screen mutant in full-length Prp8 on a TRP plasmid

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Expression

    Yeast

    Mutation

    S1584F

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS424 Prp8 S1584Y

    Plasmid

    #234121

    Purpose

    Suppressor screen mutant in full-length Prp8 on a TRP plasmid

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Expression

    Yeast

    Mutation

    S1584Y

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS424 Prp8 V1862L

    Plasmid

    #234123

    Purpose

    Suppressor screen mutant in full-length Prp8 on a TRP plasmid

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Expression

    Yeast

    Mutation

    V1862L

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge