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pY71-tet.I.10

Plasmid

#133531

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.9

Plasmid

#133530

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.7

Plasmid

#133529

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.6

Plasmid

#133528

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.5

Plasmid

#133527

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.3

Plasmid

#133525

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.2

Plasmid

#133524

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.4

Plasmid

#133526

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.1

Plasmid

#133523

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Use

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-S98 (for p24)

Plasmid

#130717

Purpose

Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-S98

Use

Tags

Hisx10 and StrepTag

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRRG312-slc1a-5

Plasmid

#99102

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea slc1a-5 (also known as excitatory amino acid transporter)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

slc1a-5 (also known as excitatory amino acid transporter) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG94-runt-1

Plasmid

#99089

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Runt-1 (also known as runt)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

Insert

Runt-1 (also known as runt) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG217-soxB

Plasmid

#99086

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea soxB (also known as soxB1-1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

soxB (also known as soxB1-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG325-Unc-9

Plasmid

#99084

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Unc-9 (also known as h.26.12f)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Unc-9 (also known as h.26.12f) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG246-MCP

Plasmid

#99083

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea MCP (also known as h.45.8g)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

MCP (also known as h.45.8g) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG208-ActR1

Plasmid

#99077

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea ActR1 (activin receptor 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

ActR1 (activin receptor 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG128-activin

Plasmid

#99075

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Activin (also known as activin 2)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Activin (also known as activin 2) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pVH009

Plasmid

#80399

Purpose

Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

3xFLAG-Venus YFP-AAV
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

C-terminal LZx domain and N-terminal 3xFLAG tag, …

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pVH015

Plasmid

#80398

Purpose

Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Anti-scaffold-ffGFP-3xFLAG
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

C-terminal 3xFLAG tag and C-terminal LZx domain

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pES007 (pTet-qacR 5/pQacA-deGFP)

Plasmid

#69036

Purpose

reporter with qacR 5 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Use

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pQacA and pTet

Available since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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