We narrowed to 10,632 results for: t7

1. pJBL7034

   Plasmid

   #133869

   Purpose

   AtzA expression plasmid

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

   Insert

   AtzA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 5FAI (EMG1)

   Plasmid

   #79519

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EMG1 (EMG1 Human)
   

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   June 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pST50Trc4-STRNDHFR

   Plasmid

   #63996

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   Strep II (STR); TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETb-pHlash

   Plasmid

   #61550

   Purpose

   Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification

   Depositor

   Carl Johnson

   Article

   Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

   Insert

   pHlash
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pST50Trc1-HISDHFR

   Plasmid

   #63935

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hec1 mCherry Plk1 K82R

   Plasmid

   #45206

   Depositor

   Michael Lampson

   Article

   Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

   Insert

   Hec1-targeted mCherry Plk1 K82R
   

   Expression

   Mammalian

   Mutation

   Plk1 K82R mutation

   Promoter

   T7

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVY-18 TC-sfGFP

   Plasmid

   #194632

   Purpose

   fluorescent protein with tetracysteine tag

   Depositor

   Allie Obermeyer

   Article

   Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

   Insert

   tetracysteine-sfGFP
   

   Tags

   MGCCPGCCGGA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b-RL018A

   Plasmid

   #42137

   Depositor

   Sheref Mansy

   Article

   Lentini et al ACS Synth Biol. 2013 Mar 15.

   Insert

   mCerulean-mCherry
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL2044

   Plasmid

   #39402

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-23-Left
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pET28b-Sad1

    Plasmid

    #90297

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW-SpyCas1

    Plasmid

    #113248

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas1
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW-SpyCas2

    Plasmid

    #113249

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas2

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas2
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSARSF505

    Plasmid

    #46832

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    GFPN-NpuDnaE_intein-GFP_C
    

    Tags

    GFP_C and H6-GFP_N

    Expression

    Bacterial

    Mutation

    stemmer GFP with superfolder mutations S30R and Y…

    Promoter

    T7

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. TAL2006

    Plasmid

    #39364

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    eGFP-TALEN-04-Left
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pST50Trc3-STRNDHFR

    Plasmid

    #63978

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. 234_pSKB3-Venus-LUMP

    Plasmid

    #65975

    Purpose

    Bacterial expression of Venus-LUMP FRET pair

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Insert

    LUMP
    

    Tags

    Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. His6_BcLOV4_Q355N_mCherry_BamUK

    Plasmid

    #119762

    Purpose

    Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

    Depositor

    Brian Chow

    Article

    Glantz et al Methods Enzymol. 2019;622:249-270. doi:

    Insert

    BcLOV4-Q355N_mCherry
    

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Mutated glutamine at position 355 to asparagine

    Promoter

    T7

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRD324

    Plasmid

    #198045

    Purpose

    Expression of HMfB D14A K30A E34A in E. coli

    Depositor

    Remus Dame

    Article

    Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

    Insert

    HMfB D14A K30A E34A
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBzCas13b

    Plasmid

    #164862

    Purpose

    Inducible expression of BzCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    Bergeyella zoohelcum Cas13b
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet11a-ABLE

    Plasmid

    #158627

    Purpose

    Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

    Depositor

    William DeGrado

    Article

    Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

    Insert

    Apixaban-Binding Helical Bundle
    

    Tags

    6x His-tag and TEV cleavage tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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