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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 13,014 results for: cdna

Showing: 6521 - 6560 of 13014 results

  7. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid
    #160742
    Purpose
    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
    Depositor
    Christina Woo
    Article
    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10
    Insert
    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    Use
    Tags
    HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    T7/CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160742-plasmid-map-sequence-id-332924

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  12. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid
    #59826
    Purpose
    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.
    Depositor
    Stephen Taylor
    Article
    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3
    Insert
    Separase (ESPL1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…
    Promoter
    hybrid human cytomegalovirus (CMV)/TetO2 promoter
    Available since
    Feb. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    59826-plasmid-map-sequence-id-99124

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  20. YFP(159-238)-gamma-1 in pcDNAI/Amp

    Plasmid
    #54471
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-1. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP(159-238)-gamma-1 (GNGT1 Aequorea victoria, Bovine)
    Use
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-1 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54471-plasmid-map-sequence-id-115002

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  25. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid
    #189562
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189562-plasmid-map-sequence-id-374127

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  29. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid
    #49065
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49065-plasmid-map-sequence-id-72190

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Showing: 6521 - 6560 of 13014 results