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sh-RNA hULK2

Plasmid

#31970

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK2 (ULK2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-BCR-ABL-p210-FLAG

Plasmid

#205619

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF73-2xCSTREP

Plasmid

#136232

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF73

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7-K122-TEAD-p-H2B-mCherry

Plasmid

#139648

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

IRES-GFP

Use

Lentiviral

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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ETV2_pet28a

Plasmid

#131665

Purpose

Bacterial expression of ETS gene ETV2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV2 (ETV2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-(SIM)1

Plasmid

#126954

Purpose

Bacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Tags

eGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLEXm-GST-ATG14

Plasmid

#99329

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

ATG14 (ATG14 Human)

Tags

GST-TEV tag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pIC292

Plasmid

#51405

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska2 IMAGE 4685972 (SKA2 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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YES1 (T348I) in pLX303

Plasmid

#42564

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T348I

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
HA-AMOT p130 deltaEYLI

Plasmid

#32826

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

E5D, deletion of last 4 amino acids (EYLI)

Promoter

CMV

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pet-15b-microID

Plasmid

#172881

Purpose

expresses microID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-GATA4

Plasmid

#101417

Purpose

Donor Vector containing GATA4 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

GATA4 (GATA4 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-30-RexYFP

Plasmid

#141070

Purpose

Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

RexYFP

Tags

6xHis

Expression

Bacterial

Mutation

changed Leucine 169 to Proline, changed Tyrosine …

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 ORF7A_nostop

Plasmid

#149324

Purpose

Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEBTet-SNAP-ALMS1

Plasmid

#136828

Purpose

Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-ALMS1 (ALMS1 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ239

Plasmid

#108859

Purpose

FnCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

Cpf1 is rice codon optimized

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC32

Plasmid

#62327

Purpose

sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-PPxY

Plasmid

#61006

Purpose

Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 PPxY mutation (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

PPxY mutation

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pSplit-ON-hDib1

Plasmid

#58787

Purpose

Express hDib1-LucN fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (1-398aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-myc-AP1S3

Plasmid

#58292

Purpose

mammalian expression of AP1S3

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Tags

6xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only