We narrowed to 62,913 results for: cloning
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Plasmid#91694PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDellaDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_JAK2_WT
Plasmid#81756PurposeGateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJH376
Plasmid#86842Purposemamalian expression of anti-CRISPR protein AcrIIA4DepositorInsertacrIIA4
ExpressionMammalianPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUBC_V5_MS2-mCherry
Plasmid#68420PurposeTransient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoterDepositorInsertMS2
UseSynthetic BiologyTagsV5 and mCherryExpressionMammalianMutationMS2: V75E/A81GPromoterhuman UbCAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Beta-Lactamase
Plasmid#62729PurposeExpresses Beta-Lactamase protein in bacterial cellsDepositorInsertBeta-Lactamase
TagsHis tagExpressionBacterialMutationDeletion of periplasmic signal domainPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJZC34
Plasmid#62331PurposesgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cellsDepositorInsertssgRNA + 2x MS2(wt+f6) binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-Lhx3
Plasmid#32927DepositorAvailable sinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223-LYN
Plasmid#23905DepositorInsertLYN (LYN Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223-PRKAA2
Plasmid#23671DepositorInsertPRKAA2 (PRKAA2 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-HA-JOSD1
Plasmid#22547DepositorPublicationAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti X2 Neo DEST (w18-1)
Plasmid#17390Purpose3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, NeoDepositorPublicationTypeEmpty backboneUseGateway Cloning, Lentiviral, and RNAiAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUC8 mouse IFN-gamma
Plasmid#10955DepositorInsertinterferon-gamma (Ifng Mouse)
UseCloning vectorAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmyc-MOV10
Plasmid#10977DepositorAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFLAG/HA-TNRC6B
Plasmid#10979DepositorAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2
Plasmid#201916PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorInsertsGag-Cas9 v2
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
ExpressionMammalianPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX304_CMV::mCherry-LgBiT-FLAG-NES
Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseExpressionMammalianPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBbS5a-Opto-Cre-Vvd-2
Plasmid#160400PurposeUpdated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd-2
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterlacUV5Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP
Plasmid#156448PurposeFor transient expression of human CTCF tagged with eGFPDepositorAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol2-HuC(elavl3)-CaMPARI2
Plasmid#137185Purposepan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)DepositorInsertHuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
UseTol2 plasmid for zebrafishTagsFLAG, HA, mycPromoterHuC(elavl3)Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRESCA-syntide
Plasmid#132415PurposeFRET-based sensor for CaMKII activityDepositorInsertFRESCA-syntide
ExpressionBacterial and MammalianPromoterCMVAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only