We narrowed to 15,050 results for: pET

1. mGreenLantern-S OR T-SpyTag

   Plasmid

   #243774

   Purpose

   Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.

   Depositor

   Cole DeForest

   Article

   Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

   Insert

   mGreenLantern
   

   Tags

   6x His tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. mGreenLantern-A AND T-SpyTag

   Plasmid

   #243776

   Purpose

   Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.

   Depositor

   Cole DeForest

   Article

   Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

   Insert

   mGreenLantern
   

   Tags

   6x His tag and SsrA

   Expression

   Bacterial

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MZB139

   Plasmid

   #217838

   Purpose

   Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Tags

   10x His and SUMO (SMT3)

   Expression

   Bacterial

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MZB164

   Plasmid

   #217842

   Purpose

   10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   HaloTag
   

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSRK51

   Plasmid

   #223722

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   Sreejith Raran-Kurussi

   Article

   Parida et al Curr Res Struct Biol. 2023 Sep 30;6:1001

   Insert

   TEV protease
   

   Tags

   NT* (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   T7

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQA-LP580

   Plasmid

   #222959

   Purpose

   Expresses R141S K142S LoaP double mutant

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 141 to Serine and Lysine 142 to …

   Promoter

   T7

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQA-LP579

   Plasmid

   #222958

   Purpose

   Expresses R36S K51S LoaP double mutant

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 36 to Serine and Lysine 51 to Se…

   Promoter

   T7

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIT203

   Plasmid

   #204512

   Purpose

   Bacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assays

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   C04F12.1 (C. elegans)
   

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIT517

   Plasmid

   #204518

   Purpose

   Bacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assays

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   cec-4 (C. elegans)
   

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Atg32 [200-341] 1B

    Plasmid

    #190431

    Purpose

    Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

    Depositor

    Michael Ragusa

    Article

    Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

    Insert

    Atg32
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLz54_polyPro_polar

    Plasmid

    #140847

    Purpose

    Same as pLz47 but with 20 polar amino acids instead of N*- peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with polar residues
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLz58_polyPro_CsoS2_CTP

    Plasmid

    #140850

    Purpose

    Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 C-terminal peptide
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLz33_CsoS2_NTD_GFP

    Plasmid

    #140861

    Purpose

    His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD fused to GFP
    

    Tags

    Hexahistidine tag and Superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLz55_polyPro_random_Npeptide

    Plasmid

    #140846

    Purpose

    Same as pLz47 but with randomized N*-peptide sequence

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with randomized CsoS2 N-peptide
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLz56_polyPro_CsoS2_MR_r1

    Plasmid

    #140848

    Purpose

    Same as pLz47 but with MR repeat 1 instead of N*-peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 MR repeat 1
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLz57_polyPro_CsoS2_CTD_r8

    Plasmid

    #140849

    Purpose

    Same as pLz47 but with CTD repeat 8 instead of N*-peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 CTD repeat 8
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWDY1226

    Plasmid

    #138593

    Purpose

    Expresses NatL2-R156A in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    changed Arginine 156 to Alanine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWDY1222

    Plasmid

    #138592

    Purpose

    Expresses NatL2-K418A in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    changed Lysine 418 to Alanine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZF-AA-GCN

    Plasmid

    #127479

    Purpose

    coding sequence for synthetic zinc finger ZF-AA-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-AA fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Promoter

    T7

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD1cR

    Plasmid

    #86970

    Purpose

    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, G108C in PCNA1

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPD1R

    Plasmid

    #86969

    Purpose

    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPD2cX

    Plasmid

    #86968

    Purpose

    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGS-3

    Plasmid

    #85094

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC3

    Plasmid

    #85087

    Purpose

    Expresses PCNA3 protein fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    PCNA3 fused to P450cam
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. Danio rerio cryaa

    Plasmid

    #46166

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. Cyprinodon variegatus cryaa

    Plasmid

    #47664

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CaMCLC-His

    Plasmid

    #111292

    Purpose

    bacterial expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    (S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase
    

    Tags

    His tag, GGGSGGGSLEHHHHHH

    Expression

    Bacterial

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Annexin A7-TEV-TwinStrep

    Plasmid

    #198635

    Purpose

    Annexin A7 fibrillization and lipid binding

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 (ANXA7 Human)
    

    Tags

    TEV-GS-Dual Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. Alpha-Synuclein-His

    Plasmid

    #186802

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    Alpha-Synuclein-His (SNCA Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMSP1D1

    Plasmid

    #20061

    Purpose

    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    deletion mutant (1-11) of MSP1; His-tag on N-term…

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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