Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 19,311 results for: MUT

Clear keywords
Showing: 3281 - 3300 of 19311 results

  1. pLenti-EML4-ALK variant 3a I1171N

    Plasmid
    #183832
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183832-plasmid-map-sequence-id-360487

    Enlarge

  2. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

    Plasmid
    #183080
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Mutation
    -124C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183080-plasmid-map-sequence-id-363419

    Enlarge

  3. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid
    #183081
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Mutation
    -146C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183081-plasmid-map-sequence-id-363415

    Enlarge

  4. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid
    #183079
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Mutation
    Wildtype promoter, Exon 1 C7C (C21>T21) silent…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183079-plasmid-map-sequence-id-363414

    Enlarge

  5. Lenti-CMV-EGFP-MTHFD1 K56R-WPRE

    Plasmid
    #250371
    Purpose
    Lentiviral expression of human MTHFD1 carrying K56R mutation with N-terminal EGFP and puromycin resistance cassette under PGK promoter. Mutation was generated by Q5 site-directed mutagenesis.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    MTHFD1 (MTHFD1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    K56R
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250371-plasmid-map-sequence-id-498985

    Enlarge

  6. Lenti-CMV-EGFP-MTHFD1 K384E-WPRE

    Plasmid
    #250372
    Purpose
    Lentiviral expression of human MTHFD1 carrying K384E mutation with N-terminal EGFP and puromycin resistance cassette under PGK promoter. Mutation was generated by Q5 site-directed mutagenesis.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    MTHFD1 (MTHFD1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    K384E
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250372-plasmid-map-sequence-id-498984

    Enlarge

  7. pLenti-EML4-ALK variant 3a G1202R

    Plasmid
    #183830
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    G1202R
    Promoter
    CMV
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183830-plasmid-map-sequence-id-360489

    Enlarge

  8. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid
    #183831
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    L1196M/G1202R
    Promoter
    CMV
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183831-plasmid-map-sequence-id-360488

    Enlarge

  9. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid
    #124257
    Purpose
    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    Expression
    Mammalian
    Mutation
    Arginine 132 is mutated to Histidine. This mutati…
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124257-plasmid-map-sequence-id-252091

    Enlarge

  10. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid
    #183833
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N/D1203N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183833-plasmid-map-sequence-id-360485

    Enlarge

  13. pCSmEn2n4ERT(#234)

    Plasmid
    #184082
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(n4)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184082-plasmid-map-sequence-id-365718

    Enlarge

  14. pCSmEn2SRERT(#231)

    Plasmid
    #184080
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(SR)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184080-plasmid-map-sequence-id-365710

    Enlarge

  15. pCSmEn25EERT(#232)

    Plasmid
    #184081
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(5E)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …
    Promoter
    sCMV+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184081-plasmid-map-sequence-id-363924

    Enlarge

  18. FR52re

    Plasmid
    #45489
    Depositor
    David Baker
    Insert
    FR52re
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    redesign of Di-I_5_FR28
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. PL2x2_5

    Plasmid
    #45491
    Depositor
    David Baker
    Insert
    PL2x2_5
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    changed E30Y of Di-IV_5_PL2x2_6
    Available Since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. FR55

    Plasmid
    #45490
    Depositor
    David Baker
    Insert
    FR55
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    changed F14L and added Gly at N-terminus of Di-I_…
    Available Since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 3281 - 3300 of 19311 results