We narrowed to 3,361 results for: actin

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Rabbit Cav 2.2 W391A pMT2

Plasmid

#58734

Purpose

expression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

Cav2.2 (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

SV40

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-AtRBX1-T7-HsSKP1-T7-AtCUL1-S

Plasmid

#228944

Purpose

Expression AtRBX1-T7, HsSKP1-T7 and AtCUL1-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtRBX1 (RBX1 Mustard Weed)
HsSKP1 (SKP1 Human)
AtCUL1 (CUL1 Mustard Weed)

Tags

S-Tag and T7

Expression

Bacterial

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6 CHIP delU-box-Myc

Plasmid

#246738

Purpose

Mammalian expression of human CHIP with U-box domain deleted, and a myc-tag on its C-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

deletion of U-box domain

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBD-PYR1

Plasmid

#241278

Purpose

Yeast two hybrid vector expressing BD-PYR1 (wild type)

Depositor

Sean Cutler

Article

Park et al Science. 2009 May 22;324(5930):1068-71.

Insert

PYR1 (PYR1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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Cav2.2 W391A pcDNA3

Plasmid

#206085

Purpose

expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-PIAL2M-sim1

Plasmid

#67908

Purpose

Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Tags

MBP

Expression

Bacterial

Mutation

E281-A496; VEDL 425-428 AAA

Promoter

P-lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMDC32B-AtTAS1c-D2-B/c-AtMIR173

Plasmid

#137885

Purpose

Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

Depositor

Alberto Carbonell

Article

Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

Inserts

AtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos

Expression

Plant

Mutation

A. thaliana TAS1c precursor sequence including a …

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET3d-SF-DCTN1-TS

Plasmid

#169375

Purpose

E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

Depositor

Bernd Bukau

Article

Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

Insert

dynactin subunit 1 isoform 1 (DCTN1 Human)

Tags

6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag

Expression

Bacterial

Mutation

Point mutations: A139T, I304T, Q322R, T516A, E577…

Available Since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-sCX3CL1

Plasmid

#164422

Purpose

AAV plasmid expressing soluble CX3CL1 (fractalkine) in retinal pigment epithelium

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

Cx3cl1 (Cx3cl1 Mouse)

Use

AAV

Expression

Mammalian

Mutation

deleted terminal 59 amino acids

Promoter

Best1

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hPMLIII

Plasmid

#103808

Purpose

BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hLaminB1

Plasmid

#103800

Purpose

BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hLaminB1 (CIB1, LMNB1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF1

Plasmid

#141188

Purpose

Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF1 (GIGYF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-fCX3CL1

Plasmid

#164421

Purpose

AAV plasmid expressing full-length CX3CL1 (fractalkine) in photoreceptors

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

Cx3cl1 (Cx3cl1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hLaminB1

Plasmid

#103799

Purpose

BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hLaminB1 (CIB1, LMNB1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hTRF1

Plasmid

#103802

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hPMLIII

Plasmid

#103807

Purpose

BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hPMLIII (CIB1, PML Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-hTRF1

Plasmid

#103801

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-LacI-tagRFP-T

Plasmid

#103810

Purpose

'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-LacI-tagRFP-T (CIB1, lacI E. coli, Mustard Weed)

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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