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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 165,343 results for: addgene

Showing: 3281 - 3300 of 165343 results

  1. lenti-sgRNA puro

    Plasmid
    #104990
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104990-plasmid-map-sequence-id-443800

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  2. lenti-sgRNA hygro

    Plasmid
    #104991
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104991-plasmid-map-sequence-id-335144

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  3. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104992-plasmid-map-sequence-id-213507

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  4. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Article
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    199344-plasmid-map-sequence-id-402934

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  5. pHis6-SUMO-g0s2

    Plasmid
    #179293
    Purpose
    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    T7
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179293-plasmid-map-sequence-id-354916

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  7. pT2/HB

    Plasmid
    #26557
    Depositor
    Perry Hackett
    Insert
    pT2/HB
    Expression
    Bacterial
    Available Since
    Dec. 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    26557-plasmid-map-sequence-id-352286

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  9. pT2/BH

    Plasmid
    #26556
    Depositor
    Perry Hackett
    Insert
    pT2/BH
    Expression
    Bacterial
    Available Since
    Oct. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    26556-plasmid-map-sequence-id-203999

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  10. pICSL4723-P1

    Plasmid
    #86173
    Purpose
    LevelP cloning Vector
    Depositor
    Mark Youles
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86173-plasmid-map-sequence-id-193313

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  11. pWPXLd

    Plasmid
    #12258
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12258-plasmid-map-sequence-id-7270

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  12. pET21a IHF

    Plasmid
    #26641
    Depositor
    Phoebe Rice
    Insert
    IHF
    Expression
    Bacterial
    Available Since
    Oct. 22, 2010
    Availability
    Academic Institutions and Nonprofits only

  13. SIN40C.SFFV.MCS.IRES.GFP

    Plasmid
    #169280
    Purpose
    Constitutive overexpression of cDNA with GFP as a reporter fluorescent protein
    Depositor
    Dirk Heckl
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    169280-plasmid-map-sequence-id-337926

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  14. pWZL Hygro

    Plasmid
    #18750
    Depositor
    Scott Lowe
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.1-MCS-EGFP_AB

    Plasmid
    #148304
    Purpose
    Mammalian Expression vector
    Depositor
    Elisa Izaurralde
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    148304-plasmid-map-sequence-id-384608

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  17. SIN40C.SFFV.MCS.IRES.dTomato

    Plasmid
    #169279
    Purpose
    Constitutive overexpression of cDNA with dTomato as a reporter fluorescent protein
    Depositor
    Dirk Heckl
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169279-plasmid-map-sequence-id-337914

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  18. Rheb WT

    Plasmid
    #24941
    Depositor
    Kunliang Guan
    Insert
    Rheb
    Use
    N/a
    Tags
    Myc
    Available Since
    Aug. 2, 2010
    Availability
    Academic Institutions and Nonprofits only

  19. pICSL01003

    Plasmid
    #174577
    Purpose
    Level 0 Acceptor for creating C-tag modules
    Depositor
    Mark Banfield
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174577-plasmid-map-sequence-id-343538

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  20. pICSL01002

    Plasmid
    #174576
    Purpose
    Level 0 Acceptor for creating N-tag modules
    Depositor
    Mark Banfield
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174576-plasmid-map-sequence-id-343537

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Showing: 3281 - 3300 of 165343 results