We narrowed to 4,074 results for: p2a

1. mouse av full length

   Plasmid

   #217809

   Purpose

   Full length mouse integrin av expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGAV (Itgav Mouse)
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SWEET11:SWEET11-2A-GFP-GUS

   Plasmid

   #172484

   Purpose

   Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

   Depositor

   Wolf Frommer

   Article

   Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

   Insert

   SWEET11 (AT3G48740 Mustard Weed)
   

   Use

   Tags

   GFP, GUS

   Expression

   Plant

   Mutation

   Promoter

   SWEET11

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-YE1-BE4max

   Plasmid

   #198546

   Purpose

   Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-DivisionRecorder

   Plasmid

   #179446

   Purpose

   Retroviral expression vector containing the DivisionRecorder cassette

   Depositor

   Ton Schumacher

   Article

   Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

   Insert

   GFP-P2A-511-CrePI-511
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NG-CGBE1

   Plasmid

   #198537

   Purpose

   Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mouse a2b full length

   Plasmid

   #217812

   Purpose

   Full length mouse integrin a2b expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGA2B (Itga2b Mouse)
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-OC-IRES-BSD

   Plasmid

   #53118

   Purpose

   To create stable cell clones with high-level expression of Cas9 and OCT1

   Depositor

   Wensheng Wei

   Article

   Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Inserts

   OCT1 (POU2F1 Human)
   Cas9
   

   Use

   Lentiviral

   Tags

   IRES-BSD, NLS, and P2A

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSK+ chickBMPR-1B DN

   Plasmid

   #49529

   Purpose

   cloning vector for dominant negative chick BMP receptor 1B

   Depositor

   Peter ten Dijke, Lee Niswander

   Article

   Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

   Insert

   BMPR-1B DN (BMPR1B Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   dominant negative mutation (K231R); also P2A, M11…

   Promoter

   T3

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Sniper-Cas9

    Plasmid

    #138559

    Purpose

    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    Sniper-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

    Plasmid

    #184952

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Ami Bhatt, Michael Bassik, Patrick Hsu, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid

    #168106

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    mCherry-KASH
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL-CRISPR.SFFV.PAC

    Plasmid

    #57829

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-synP-FLEX-splitTVA-EGFP

    Plasmid

    #52474

    Purpose

    Expresses splitTVA-EGFP

    Depositor

    Ian Wickersham

    Article

    Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

    Insert

    splitTVA-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin-1

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid

    #169737

    Purpose

    Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLC-Flag-CSNK1A1 G40N-Puro

    Plasmid

    #123320

    Purpose

    Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Glycine 40 to Asparagine

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. FOR human NANOS3 TALEN

    Plasmid

    #59722

    Purpose

    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. REV human NANOS3 TALEN

    Plasmid

    #59723

    Purpose

    TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. human a5 full length

    Plasmid

    #217818

    Purpose

    Full length human integrin a5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA5 (ITGA5 Human)
    

    Use

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-NLS-FRB-VP16

    Plasmid

    #210515

    Purpose

    expresses NLS-FRB-VP16 component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NLS-FRB-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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