We narrowed to 4,940 results for: puc

1. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0040-LwaCas13a crRNA backbone

   Plasmid

   #103851

   Purpose

   Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-LwaCas13aDR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TCARZ-CMV-Cherry-PAmin-GF3-R4A22

   Plasmid

   #203770

   Purpose

   For production of template RNA for use in PRINT, mCherry under CMV promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for mCherry
   

   Use

   Ivt template

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT15489_dABE8e

   Plasmid

   #174127

   Purpose

   plasmid expressing ABE8e with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dABE8e
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEED-mScarlet3:ALFA

   Plasmid

   #219641

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet:ALFA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEHlyA5

   Plasmid

   #168298

   Purpose

   Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

   Depositor

   Luis Ángel Fernández

   Article

   Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

   Insert

   N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
   

   Use

   Tags

   E-tag, HA-tag, His-tag, Prescission site, and VHH…

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE332

   Plasmid

   #153241

   Purpose

   M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGE333

   Plasmid

   #153242

   Purpose

   M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK305

   Plasmid

   #66812

   Purpose

   IVT vector for Fluc mRNA

   Depositor

   Ron Weiss

   Article

   Andries et al J Control Release. 2015 Sep 2. pii: S016

   Insert

   Firefly luciferase
   

   Use

   Luciferase; Mrna ivt

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE335

    Plasmid

    #153244

    Purpose

    M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

    Plasmid

    #214989

    Purpose

    Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTPH418b

    Plasmid

    #185725

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT15482_ABE8e

    Plasmid

    #174120

    Purpose

    Expresses ABE8e with an EF1a promoter

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AICSDP-60: SOX2-mEGFP

    Plasmid

    #124606

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2

    Depositor

    Allen Institute for Cell Science

    Insert

    SOX2 Homology Arms with linker-mEGFP (SOX2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pNG1299

    Plasmid

    #149709

    Purpose

    Template for Malachite Green Aptamer (MGA) transcription assay

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    Triple Promoter - 12X MG Aptamer Array - Terminator construct
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-C-mScarlet-EFS-Blast

    Plasmid

    #227260

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHBJT001

    Plasmid

    #225152

    Purpose

    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGE655

    Plasmid

    #153253

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD268

    Plasmid

    #132523

    Purpose

    mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mNG-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mNG

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCP-tRNA

    Plasmid

    #133812

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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