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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,613 results for: SHA

Showing: 3321 - 3340 of 11613 results

  1. pNK845

    Plasmid
    #219746
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219746-plasmid-map-sequence-id-437111

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  2. PCDF Bravo-PotH-OL3/2sub

    Plasmid
    #216752
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Article
    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi
    Insert
    potH (potH E. coli (Bacteria))
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 2 (OL2) with the sequence of [WMGILKNN…
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216752-plasmid-map-sequence-id-434424

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  4. pCEP4 TAPBPR-TM-TN6

    Plasmid
    #178650
    Purpose
    Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)
    Depositor
    Erik Procko
    Article
    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038
    Insert
    TAPBPR (TAPBPL Human)
    Use
    Tags
    FLAG and Signal/leader sequence from HLA class I …
    Expression
    Mammalian
    Mutation
    E205K, R207E, Q209S, Q272S, switched transmembran…
    Promoter
    CMV
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    178650-plasmid-map-sequence-id-354770

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  7. pGEX2T-TEV CHMP1B 4-199 L188A/L192A

    Plasmid
    #108289
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108289-plasmid-map-sequence-id-245274

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  8. pGEX2T-TEV CHMP1B 4-199 L192A/L195A

    Plasmid
    #108291
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 192 to A, changed L 195 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108291-plasmid-map-sequence-id-245276

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  10. AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610)

    Plasmid
    #191737
    Purpose
    AI plasmid ALIAS: AiP12610. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    191737-plasmid-map-sequence-id-376686

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  11. AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237)

    Plasmid
    #191707
    Purpose
    AI plasmid ALIAS: AiP12237. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    191707-plasmid-map-sequence-id-376693

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  12. AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646)

    Plasmid
    #230884
    Purpose
    AiE1426m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Sst-Chodl neurons
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230884-plasmid-map-sequence-id-459521

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  14. AiP13808 - pAAV-AiE0140h_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3808)

    Plasmid
    #224176
    Purpose
    AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neurons (note cortical Pvalb+ and L5_ET)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Insert
    mTFP1
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    224176-plasmid-map-sequence-id-446818

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  16. AiP12700 - pAAV-AiE0452h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2700)

    Plasmid
    #191708
    Purpose
    AI plasmid ALIAS: AiP12700. Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Insert
    mTFP1
    Use
    AAV
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    Feb. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    191708-plasmid-map-sequence-id-376694

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  17. AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)

    Plasmid
    #199777
    Purpose
    enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mutation
    R297T for reduced recombinase efficiency.
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    199777-plasmid-map-sequence-id-391721

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Showing: 3321 - 3340 of 11613 results