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pMXs-ERas CVLS-IP

Plasmid

#13828

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas CVLS (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

CSVA (224-227) changed to CVLS (CAAX sequence of …

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pCI-MZAS

Plasmid

#51872

Purpose

Expresses N-terminal FLAG-tagged rat MIBP1 (amino acids 1721-2155)

Depositor

Kenshi Hayashi, Tomoko Tahira

Article

Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

Insert

MIBP1 (Hivep2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

amino acids 1721-2155

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn1-iCre-P2A-mRuby3

Plasmid

#235248

Purpose

AAV-mediated expression of iCre and mRuby3 in mammalian cells

Depositor

Takeshi Imai

Article

Egashira et al Sci Adv. 2026 Jan 16;12(3):eadw8458. doi

Inserts

iCre
mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hsyn1

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-jGCaMP8m-P2A-CyRFP1-WPRE

Plasmid

#235249

Purpose

AAV-mediated and Cre-dependent expression of jGCaMP8m and CyRFP1 in mammalian cells

Depositor

Takeshi Imai

Article

Egashira et al Sci Adv. 2026 Jan 16;12(3):eadw8458. doi

Inserts

jGCaMP8m
CyRFP1

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pET21a Di-iPGM

Plasmid

#180241

Purpose

Bacterial expression plasmid for production of recombinant Dirofilaria immitis iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Dirofilaria immitis iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_NPM2-T2A-mGreenLantern-PuroTK

Plasmid

#222910

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_FIGLA-T2A-mGreenLantern-PuroTK

Plasmid

#222905

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

Plasmid

#222915

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZGLP1

Plasmid

#222926

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZGLP1 (ZGLP1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-SOHLH1

Plasmid

#222922

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOHLH1 (SOHLH1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-1700029I15Rik-3×FLAG

Plasmid

#199689

Purpose

Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

Depositor

Masahito Ikawa

Article

Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

Insert

1700029I15Rik (1700029I15Rik Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRA1SEGFPTcIfm

Plasmid

#193777

Purpose

Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators

Use

Synthetic Biology

Expression

Bacterial

Promoter

PR promoter and PLTetO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pKI-cjT-tdTomato-PGK-Neo

Plasmid

#186173

Purpose

Knock-in gene targeting in marmoset cells at TBXT locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjOSR1-EGFP-PGK-Puro

Plasmid

#186170

Purpose

Knock-in gene targeting in marmoset cells at OSR1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-tdTomato-PGK-Neo

Plasmid

#186172

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-hCD271-PGK-Neo

Plasmid

#186171

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

hCD271

Use

Marmoset targeting

Mutation

human codon-optimized

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-EGFP-PGK-Puro

Plasmid

#186167

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3R2

Plasmid

#179163

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mPpp3r2

Plasmid

#179161

Purpose

Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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mYF

Plasmid

#171460

Purpose

Expresses mYF, a CiCE of NiCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

EYFP(A207K)-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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