We narrowed to 14,145 results for: TIM
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Plasmid#248886PurposeEdit Expresses 6xHis-tagged C-terminal domain of TDP-43 with M339A.DepositorAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only
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TDP-43_CTD_L340A
Plasmid#248887PurposeEdit Expresses 6xHis-tagged C-terminal domain of TDP-43 with L340A.DepositorAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-MLLn-CAGEn-NES (Nrdj1)
Plasmid#241412PurposeProximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.DepositorAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-MLLn-CAGEn-NES (Npu)
Plasmid#241411PurposeProximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.DepositorAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-AMLn-CAGEn-NES (Nrdj1)
Plasmid#241408PurposeProximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Figure 2 in Nature Chemical Biology 20.10 (2024): 1353-1360.DepositorAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CAG-NGN3-linker-3xFLAG-Dendra2-HA
Plasmid#234887PurposeExpresses human NGN3 in mammalian cells directly fused to Dendra2 fluorescent protin plus FLAG and HA tagsDepositorInsertNGN3-3xFLAG-Dendra2-HA (NEUROG3 Human)
Tags3xFLAG, Dendra2 and HAExpressionMammalianPromoterCAGAvailable SinceSept. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-AMLn-CAGEn-NES (Npu)
Plasmid#241407PurposeProximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Extended Figure 3 in Nature Chemical Biology 20.10 (2024): 1353-1360.DepositorAvailable SinceSept. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_G-PTEN
Plasmid#227432PurposeExpresses the G-PTEN sensor under a CMV promoterDepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBC001 v3
Plasmid#161711PurposeCMVp-EGFP-[barcode cloning site]-PGKp-Puro-WPREDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyExpressionMammalianPromoterCMV PromoterAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Donor-attp-u6-blank-sgrna-backbone
Plasmid#234076PurposeBlank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 lociDepositorInsertsgRNA-Blank
UseLentiviralExpressionMammalianAvailable SinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_R-PTEN_4A
Plasmid#227435PurposeExpression of the R-PTEN sensor with a 4A mutationDepositorAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CAG_G-PTEN
Plasmid#227436PurposeExpresses the G-PTEN sensor under a CAG promoterDepositorInsertG-PTEN sensor, mEGFP-PTEN-sREACh (Pten Rat)
TagsmEGFP, and sREAChExpressionMammalianMutationR14GPromoterCAGAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX330-PTEN
Plasmid#227440PurposeCas9 expression and a gRNA targeting Exon 6 of mouse PTENDepositorInsertspCas9 and gRNA for mouse PTEN (Pten )
ExpressionMammalianAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_G-PTEN_4A
Plasmid#227434PurposeExpresses the G-PTEN sensor with a 4A mutationDepositorInsertG-PTEN 4A (Pten Rat)
TagsmEGFP and sREAChExpressionMammalianMutation4A mutation.PromoterCMVAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCpep-E1GFP
Plasmid#233884PurposeExpression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granulesDepositorInsertProinsulin-E1GFP (Ins2 Mouse)
ExpressionMammalianMutationInserted E1GFP into C-peptide of mouse Ins2 codin…PromoterCMVFAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 L328D, L355A (1-366)
Plasmid#193040PurposeBacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.DepositorAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 V18D-OSF
Plasmid#193042PurposeMammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationV18DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 L61D-OSF
Plasmid#193043PurposeMammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationL61DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAPN7 F98D-OSF
Plasmid#193044PurposeMammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
TagsOSF (One-Strep-FLAG)ExpressionMammalianMutationF98DPromoterChicken Beta-actinAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-V18D-mCherry
Plasmid#193046PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationV18D, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CAPN7-C290S-mCherry
Plasmid#193047PurposeLentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.DepositorInsertCAPN7 (CAPN7 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationC290S, siRNA resistant to: GCACCCAUACCUUUACAUUPromoterTight TREAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 V18D,F98D (1-165)
Plasmid#193034PurposeBacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.DepositorInsertCAPN7 (CAPN7 Human)
Tags6xHis-SumoExpressionBacterialMutationV18D, F98D, Residues 1-165PromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PD2529 human beta1 ecto
Plasmid#221398PurposeMammalian expression of human integrin beta1 ectodomainDepositorInsertintegrin beta1 ectodomain (ITGB1 Human)
TagsAVI tag, HRV3C cleavage site, basic coil, HA tag,…ExpressionMammalianMutationcodon optimized for human mature _1 residues Q1 t…Available SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28(CD45TM)
Plasmid#223629PurposeLentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPMutationCD28 transmembrane domain replaced by CD45 transm…Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28(CD45TM)
Plasmid#223624PurposeLentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsSpytag003MutationCD28 transmembrane domain replaced by CD45 transm…Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-CD86(F56A)
Plasmid#223609PurposeLentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-CD80(Y65A)
Plasmid#223608PurposeLentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28(SGGG)
Plasmid#223607PurposeLentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPMutationMYPPPY mutated to SGGGAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CTLA-4-GCN4-His6
Plasmid#223582PurposeProtein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD80 (I92R)-His10
Plasmid#223580PurposeProtein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD80-His10
Plasmid#223579PurposeProtein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-eSpCas9(1.1)
Plasmid#178800PurposeHigh-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3-SpCas9-HF1
Plasmid#178801PurposeHigh-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG001-ACE2(WT)-HA
Plasmid#191569PurposeACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.DepositorInsertAngiotensin Converting Enzyme II (ACE2 Human)
UseLentiviral and RNAiTagsHA tagExpressionMammalianMutationCodon optimized for expression in human cellsPromoterCMVAvailable SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Beta1 Q280* K418* full-length
Plasmid#221406PurposeMammalian expression of human integrin beta1 Q280* K418* full-lengthDepositorInsertintegrin beta1 Q280* K418* full-length (ITGB1 Human)
TagsCD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …ExpressionMammalianMutationcodon-optimized mature sequenceAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Beta1 N391* N562* full-length
Plasmid#221407PurposeMammalian expression of human integrin beta1 N391* N562* full-lengthDepositorInsertintegrin beta1 N391* N562* full-length (ITGB1 Human)
TagsCD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …ExpressionMammalianMutationcodon-optimized mature sequenceAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-HT(C199S)_WPRE
Plasmid#216415PurposeEncodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectorsDepositorInsertoROS-HT(C199S)
UseAAVExpressionMammalianPromoterCAGAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZIC3_5-5)-PGKpuroBFP-W
Plasmid#211997PurposeExpress gRNA against ZIC3 with puro and BFPDepositorInsertsgRNA targeting ZIC3 (ZIC3 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZIC3_5-3)-PGKpuroBFP-W
Plasmid#211996PurposeExpress gRNA against ZIC3 with puro and BFPDepositorInsertsgRNA targeting ZIC3 (ZIC3 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(TRIM24_4)-PGKpuroBFP-W
Plasmid#211988PurposeExpress gRNA against TRIM24 with puro and BFPDepositorInsertsgRNA targeting TRIM24 (TRIM24 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterHuman U6 promoterAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only