We narrowed to 3,483 results for: mpo

1. pcDNA3.1-Beta4

   Plasmid

   #166773

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB4 (GNB4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. N-Flag-IPO9_Lenti SFFV Puro WPRE

   Plasmid

   #217916

   Purpose

   Express IPO9 in mammalian cells

   Depositor

   Benjamin Cravatt

   Article

   Ogasawara et al Cell Chem Biol. 2024 Nov 7:S2451-9456(24

   Insert

   importin 9 (IPO9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-GGamma8-GFP2

   Plasmid

   #140990

   Purpose

   Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma8-GFP2 (GNG8 Human)
   

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221 UBR5 ∆UBR

   Plasmid

   #81063

   Purpose

   Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
   

   Use

   Cloning

   Mutation

   W1235L (bp g3704t)

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR221 UBR5 ∆HECT

   Plasmid

   #81065

   Purpose

   Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
   

   Use

   Cloning

   Mutation

   C2768A (bp t8302g and g8303c)

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-AMVpl-δWT-nAchR

   Plasmid

   #97188

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Delta nicotinic acetylcholine receptor (Chrnd Mouse)
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-AMVpl-γWT-nAchR

   Plasmid

   #97187

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Gamma nicotinic acetylcholine receptor (Chrng Mouse)
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPR77-DuET

   Plasmid

   #213291

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR77 (C5AR2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SHC003BSD-EXOC7

   Plasmid

   #133298

   Purpose

   Expresses EXOC7 in mammalian cells

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Insert

   Exocyst complex component 7 (EXOC7 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MT-AvrXa10-FT

    Plasmid

    #234373

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

    Depositor

    Michael Smanski

    Article

    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

    Inserts

    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    

    Use

    Synthetic Biology

    Tags

    GB1, GFP, and GP41

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. XLone-BSD Cas13d P2A mCherry

    Plasmid

    #155183

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    CasRx
    

    Use

    Unspecified; Piggybac transposon

    Tags

    NLS and P2A mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Venus-Puma-pEGFP-C1

    Plasmid

    #166739

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Puma (BBC3 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid

    #68218

    Purpose

    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc / Renilla / P19
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos / 35S / 35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

    Plasmid

    #117662

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pOst1-pro-af(MUT1)-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. XLone-Puro Cas13d-eGFP U6 SOX17 g2

    Plasmid

    #155188

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA2
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV NucleoBAS N354K-I372V

    Plasmid

    #63574

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS N354K-I372V
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    N345K and I372V

    Promoter

    CMV

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

17. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121817

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid

    #121814

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CreLite

    Plasmid

    #131785

    Purpose

    AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC-P2A-PIF6CreN
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-CCre-MBP4-WPRE

    Plasmid

    #193919

    Purpose

    Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP4
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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