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pFl Strep-sumo-TEV-hAgo2

Plasmid

#136235

Purpose

Expresses human Argonaute-2 in insect cells

Depositor

Leemor Joshua-Tor

Article

Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

Insert

human Argonaute-2 (AGO2 Human)

Tags

Strep Sumo

Expression

Insect

Promoter

polyhedrin

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-mKeima-LGALS3

Plasmid

#175780

Purpose

Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-rAPOBEC1-pAG

Plasmid

#248102

Purpose

Expresses rAPOBEC1 fused to N-terminus of Protein A/G in insect cells

Depositor

Yangming Wang

Article

Cheng et al Nat Methods. 2025 Sep;22(9):1824-1835. d

Inserts

rAPOBEC1 (Apobec1 Rat)
protein A/G

Tags

His and Strep

Expression

Insect

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pCAX APP C99

Plasmid

#30146

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

APP C99 (APP Human)

Expression

Mammalian

Mutation

C-terminal from BACE site of APP

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-2xHA-MOF

Plasmid

#29763

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

MYST1 (KAT8 Human)

Tags

2xHA

Expression

Insect

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Mutation

See depositor comments below.

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-Bcl2

Plasmid

#46971

Purpose

lentiviral expression of Bcl2

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

Bcl2 (BCL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43

Plasmid

#60691

Purpose

mammalian expression of rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pIRES proHB-EGF WT

Plasmid

#11602

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3/Flag-METTL3

Plasmid

#53739

Purpose

For expression of METTL3 (methyltransferase-like 3) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 3 (METTL3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-Wild-Type

Plasmid

#235169

Purpose

Expresses Wild-Type GRP78 in mammalian cells

Depositor

Amy Shiu Lee

Article

Zhang et al J Biol Chem. 2010 May 14;285(20):15065-1

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-DNAseI

Plasmid

#182409

Purpose

Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DNase I (DNASE1 Bovine)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PWPXLD-EGFR-WT

Plasmid

#133749

Purpose

Wild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimd

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

Wild type EGFR-HA (EGFR Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3

Plasmid

#175778

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ14902

Plasmid

#239277

Purpose

Expresses gG2 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PspCas13b-hGAPDH-gRNA2 (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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T7-p53DD-pcDNA3

Plasmid

#25989

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p53 (TP53 Human)

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 300-…

Available Since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pCAX APP 751

Plasmid

#30138

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Expression

Mammalian

Mutation

wild type splice variant 751

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

Plasmid

#238184

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 (KLF5 Synthetic, Human)

Use

Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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