We narrowed to 10,333 results for: SEC

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SAE2-mGarnet

Plasmid

#175240

Purpose

Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBA2 (UBA2 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585

Plasmid

#174401

Purpose

S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag

Plasmid

#179715

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-CSF2-218-PDGFR

Plasmid

#139058

Purpose

A lentivirus vector to integrate a surface membrane anchored human CSF2 gene

Depositor

Tao Liu

Article

Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

Insert

colony stimulating factor 2 (CSF2) (CSF2 Human)

Use

Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-erHRP(N175S mutant)

Plasmid

#79909

Purpose

erHRP(N175S mutant) pCMV backbone

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP(C3)-Nup153-C (880-1475)

Plasmid

#64317

Purpose

GFP-tagged mammalian expression of C-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Tags

GFP

Expression

Mammalian

Mutation

C-terminal subunit (880-1475)

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pAB092a

Plasmid

#79217

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1pr_v2-RT

Plasmid

#97088

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1pr_v2-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAB078d8

Plasmid

#79206

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-pCaMKII-EBFP-tTAn

Plasmid

#172124

Purpose

Expression of EBFP and tTA-InteinN in CaMKII positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, EBFP, tTAn-InteinN

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pYSD099

Plasmid

#212923

Purpose

A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

GFP

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E S209D IRES GFP

Plasmid

#18765

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E S209D (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

S209D

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pCDH-hSyn-NCreIntN-T2A-mTagBFP2

Plasmid

#160502

Purpose

Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pVk7-33G1-Flag

Plasmid

#58950

Purpose

backbone for mouse recombinant antibody with added C-terminal FLAG tag

Depositor

Gavin Wright

Article

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1m_v1-RT

Plasmid

#97089

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1m_v1-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1_IS_RT

Plasmid

#97091

Purpose

Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1_IS_RT (NEAT1 Human)

Use

CRISPR

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAB078d3

Plasmid

#79203

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pVk7-33G1-BioLHis

Plasmid

#58949

Purpose

backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

Depositor

Gavin Wright

Article

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Tags

6His tag and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-T2A-mTagBFP2

Plasmid

#160500

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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