We narrowed to 6,040 results for: WUS;
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Plasmid#126512PurposeOptopatch4. Simultaneous optical recording and manipulation of electrical activity in neuronsDepositorInsertsomArchon1-EGFP-P2A-somCheRiff
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-5-SV40 polyA
Plasmid#190869PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode5
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin S3E
Plasmid#50858Purposeexpresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 S3E (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationS3E psuedo-phosporylated, non-activatablePromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 SV40-CMV-SaCas9-3xNLS
Plasmid#78601PurposeAAV vector containing SaCas9DepositorInsertSV40-CMV-SaCas9-3xNLS
UseAAV and CRISPRTagsHA tagExpressionMammalianMutationPromoterSV40, CMV promotersAvailable sinceDec. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet2 CD
Plasmid#72219PurposeExpress flag-tagged mouse Tet2 catalytic domain.DepositorInsertTen-Eleven Translocation 2 (Tet2 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET2 catalytic domain.PromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MAPK14-P2A-Hygro_Barcode
Plasmid#170239PurposeBarcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAPK14 (MAPK14 Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124431PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafishDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseZebrafish (if expression level is too high in zeb…TagsExpressionMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 U6-SaDMDR7-U6-SaDMDL2
Plasmid#78603PurposeAAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23DepositorInsertU6-SaDMDR7-U6-SaDMDL2
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF4_P2A_Hygro_Barcode
Plasmid#120455PurposeBarcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF4 (KLF4 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic Fyn FRET Biosensor
Plasmid#124654PurposeFRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPetDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-CFP
Plasmid#193357PurposeSelf-replicating RNA vector expressing CFP fluorescent protein + PuroRDepositorInsertCFP
UseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterT7Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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MiniCoopR mitfa:KIT L576P
Plasmid#118848PurposeExpresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertKIT L576P (KIT Zebrafish, Human)
UseZebrafish expression, tol4TagsExpressionMutationKIT L576PPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCA13-pHR-SFFV-HaloTag-FBL
Plasmid#199444PurposeExpression of HaloTag-FBL in mammalian cellsDepositorInsertFBL (FBL Human)
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterSFFVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCX-CD28 WT
Plasmid#223640PurposeRetroviral expression of mouse CD28 in mammalian cellsDepositorInsertCD28 (Cd28 Mouse)
UseRetroviral; mammalian expressionTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO
Plasmid#204342PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-s/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
aCat DeltaTS (delta570 to 670 aa in aCat TS WT)
Plasmid#101300PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-509, 670-698, and 699-906 …PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S513A/S515A/S516A
Plasmid#186835PurposeExpresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 513/515/516 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3-Y130F
Plasmid#113428PurposeExpresses hA3A-BE3-Y130F in mammalian cellsDepositorInserthA3A-BE3-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationhAPOBEC3A_Y130FPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTY-EF1a-mKdm2b (del CxxC domain)-Flag
Plasmid#61740PurposeExpress CxxC domain deleted mouse histone demethylase Kdm2bDepositorInsertKdm2b (Kdm2b Mouse)
UseLentiviralTagsFlagExpressionMutationdelete aa 588-624PromoterEF1aAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only