We narrowed to 8,758 results for: app

1. pRK010

   Plasmid

   #69874

   Purpose

   expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

   Depositor

   Georg Nagel, Robert Kittel

   Article

   Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

   Insert

   Channelrhodopsin-2
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   D156C (XXL)

   Promoter

   Drosophila Hsp70

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV PIG Myb

   Plasmid

   #66988

   Purpose

   Retroviral expression plasmid for mouse Myb expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Myb (Myb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKB01_mOrange2

   Plasmid

   #69102

   Purpose

   Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mOrange2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

   Plasmid

   #202719

   Purpose

   Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Myr-Pink Flamindo
   EGFP-bPAC-CAAX
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PECAM-H2B-GFP

   Plasmid

   #14689

   Depositor

   Victoria Bautch

   Article

   Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

   Insert

   PECAM promoter/enhancer (Pecam1 Mouse)
   

   Use

   Tags

   H2B-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

7. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Promoter

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC-N-GFP

   Plasmid

   #172974

   Purpose

   MAC-tagged GFP expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   GFP
   

   Use

   Tags

   MAC-tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-L2T1L

   Plasmid

   #33287

   Depositor

   Terence Dermody

   Article

   Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

   Insert

   L2 (L2 Orthoreovirus T1L)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX4T1-TRAF6-C70A

    Plasmid

    #43930

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    TNF Receptor-Associated Factor 6 (Traf6 )
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C70A

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-LifeAct-Venus

    Plasmid

    #87613

    Purpose

    Actin-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-Venus
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCB-CBEv1

    Plasmid

    #221138

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

    Plasmid

    #71811

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-FKBP-SAC1C389S

    Plasmid

    #108128

    Purpose

    Recruitable inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(C389S)

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCellFree_KA1_ZIKV NS5

    Plasmid

    #145348

    Purpose

    Cell-free/ mammalian expression vector for Zika virus NS5 protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS5
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available Since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FAM19A2-V5

    Plasmid

    #184338

    Purpose

    Expression of V5-tagged mouse FAM19A2

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    FAM19A2 (Tafa2 Mouse)
    

    Use

    Tags

    V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7-RelA(S276A)

    Plasmid

    #24153

    Depositor

    Warner Greene

    Article

    Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

    Insert

    RelA (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    S276A

    Promoter

    Available Since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pLP-mScarlet-I

    Plasmid

    #209993

    Purpose

    Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXNubWtgate

    Plasmid

    #105080

    Purpose

    Yeast mating-based Split Ubiquitin Assay, positive control

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubI

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. BB3rN_14*

    Plasmid

    #98550

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Brigitte Gasser, Michael Sauer, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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