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pBFC0625
Plasmid#186691PurposeVcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargoDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_lacZ_α_1 Spacer crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
UseTagsExpressionBacterialMutationPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-Truncated-Kir3.2-StreptagII
Plasmid#177263PurposeExpresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cellsDepositorInsertG-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
UseTagsStreptagIIExpressionInsectMutationResidues 49-378Promoterpolyhedrin promoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFC1207
Plasmid#186694PurposeVcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right EndDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
UseTagsExpressionBacterialMutationPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRT8
Plasmid#68385PurposePHA metabolic engineeringDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRT9
Plasmid#68386PurposePHA metabolic engineeringDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRT15
Plasmid#68389PurposePHA metabolic engineering, similar to pJRT8 with R388 oriTDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
p42Neo 1-F GW
Plasmid#58550PurposeMulticopy with G418 resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p42Neo 3-F GW
Plasmid#58558PurposeMulticopy with G418 resistance marker for triple Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p41Neo 1-F GW
Plasmid#58545PurposeCEN vector with G418 resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only
