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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,422 results for: ttl

Showing: 321 - 340 of 3422 results

  5. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Expression
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

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  9. pPB9P3

    Plasmid
    #210034
    Purpose
    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Article
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    prime editor 2
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210034-plasmid-map-sequence-id-414000

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  12. Anti-Gs protein, alpha subunit [N192/12]

    Plasmid
    #190302
    Purpose
    Mammalian Expression Plasmid of anti-Gs protein, alpha subunit (Cattle). Derived from hybridoma N192/12.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Gs protein, alpha subunit (Bos taurus) recombinant Mouse monoclonal antibody (GNAS Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    190302-plasmid-map-sequence-id-372838

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  13. pP150

    Plasmid
    #113201
    Purpose
    shuttle vector, N-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Dictyostelium discoideum shuttle vector, n-termin…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    113201-plasmid-map-sequence-id-217569

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  14. pENTR_mCherry

    Plasmid
    #139554
    Purpose
    mCherry ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139554-plasmid-map-sequence-id-274727

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  15. pENTR-rtTA3G

    Plasmid
    #139556
    Purpose
    rtTA ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    rtTA3G
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139556-plasmid-map-sequence-id-274724

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  16. pENTR-Emerald

    Plasmid
    #139549
    Purpose
    Emerald ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Emerald
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139549-plasmid-map-sequence-id-274625

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  17. pENTR-TetR

    Plasmid
    #139558
    Purpose
    TetR ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    TetR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139558-plasmid-map-sequence-id-274733

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  18. pENTR-Venus

    Plasmid
    #139551
    Purpose
    Venus ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139551-plasmid-map-sequence-id-274619

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  19. pENTR-tTAF2

    Plasmid
    #139557
    Purpose
    tTAF2 ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    tTAF2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139557-plasmid-map-sequence-id-274731

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  20. pENTR-DsRedT4

    Plasmid
    #139550
    Purpose
    DsRedT4 ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    DsRedT4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139550-plasmid-map-sequence-id-274631

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Showing: 321 - 340 of 3422 results