Addgene

We narrowed to 1,605 results for: tyr

Showing: 641 - 680 of 1605 results

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pcFLAG-CSK-WT

Plasmid

#74502

Purpose

Overexpression of CSK in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-2TK cyclin T1 (P#468)

Plasmid

#14607

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (CCNT1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pRK5 beta4

Plasmid

#16037

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1-ALIX(P801G)

Plasmid

#180025

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)

Use

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mycRSK2wt

Plasmid

#15827

Depositor

Jing Chen

Article

Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

Insert

Ribosomal S6 kinase 2 (Rps6ka3 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

wild type

Promoter

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TIE-V5/HIS

Plasmid

#205612

Purpose

Expression of human TIE receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TIE receptor tyrosine kinase (TIE1 Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Dynamin-I-K44A-6His/Flag

Plasmid

#32696

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Use

Tags

Flag and His6

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pcpAzpaRS_v2

Plasmid

#196483

Purpose

To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40(synthetase), Pol III tRNA promoter (tRNA), C…

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ALIX(1-702)-mNeonGreen

Plasmid

#191193

Purpose

Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (1-702) (PDCD6IP Human, Synthetic)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Deletion of ALIX C-terminal PRD.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-puro SHP2 WT

Plasmid

#8329

Depositor

Ben Neel

Article

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_WT RAD23B

Plasmid

#201439

Purpose

Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat5A-SH2

Plasmid

#46515

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5A (STAT5A Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 582-693

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat5B-SH2

Plasmid

#46516

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5B (STAT5B Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 593-692

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX ShcD-SH2

Plasmid

#46488

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcD (SHC4 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 512-623

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL5.0 hCortKD EGFP

Plasmid

#187267

Purpose

Lentiviral expression of shRNA targeting Cortactin

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

shRNA for Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat6-SH2

Plasmid

#46517

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat6 (STAT6 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 510-654

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SH2-B-SH2

Plasmid

#46479

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B (SH2B1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Tec-SH2

Plasmid

#46522

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tec (TEC Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 236-351

Promoter

Tac

Available since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-ING1a

Plasmid

#79069

Purpose

Expression of p47ING1 in mammalian cells

Depositor

Karl Riabowol

Article

Soliman et al Aging Cell. 2008 Dec;7(6):783-94. doi: 1

Insert

ING1 (p47 ING1A isoform) (ING1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (kinase dead)

Plasmid

#29533

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
MAC_C_FGFR2

Plasmid

#187751

Purpose

MAC-tagged gene expression of human FGFR2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

FGFR2 (FGFR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-ROR1-V5/HIS

Plasmid

#205607

Purpose

Expression of human ROR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR1 receptor tyrosine kinase (ROR1 Human)

Use

Tags

V5-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF1-ERBB4 (kinase dead)

Plasmid

#29544

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
Dr-EGFR

Plasmid

#184004

Purpose

Dr-EGFR expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

Insert

Dr-EGFR (EGFR Human)

Use

Tags

Deinococcus radiodurans bacterial phytochrome

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Txk-SH2

Plasmid

#46526

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Txk (Txk Mouse)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 138-258

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHMT_hnRNPF_deltaPLD

Plasmid

#139115

Purpose

express FL hnRNPF without the prion like domain with N-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Use

Tags

Expression

Bacterial

Mutation

delta365-415

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Sh3bp2-SH2

Plasmid

#46484

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Sh3bp2 (SH3BP2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 450-561

Promoter

Tac

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Yes-SH2

Plasmid

#46532

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Yes (YES1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 152-261

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSVE Grb2 myc 49L/203R

Plasmid

#26087

Depositor

Bob Weinberg

Article

Egan et al Nature. 1993 May 6. 363(6424):45-51.

Insert

Grb2 49L/203R (GRB2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

49L/203R

Promoter

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
MAC_C_ERBB2

Plasmid

#187776

Purpose

MAC-tagged gene expression of human ERBB2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ERBB2 (ERBB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Dynamin I-K44A-non-tagged

Plasmid

#32695

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Use

Tags

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
H446 EF1a RA-ABE6.3 p2A Puro

Plasmid

#139104

Purpose

Lentiviral expression of ABE6.3.

Depositor

Wen Xue

Article

Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

Insert

ABE RA6.3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1-PRD(800-868)-strep

Plasmid

#180023

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)

Use

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPA-fapO-RgTALsyn

Plasmid

#100946

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

GAP (Constitutive)

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Supt6h-SH2

Plasmid

#46518

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Supt6h (SUPT6H Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1317-1441

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcFLAG-CSK-R107E

Plasmid

#74503

Purpose

Overexpression of CSK R107E mutant in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R107E, K222R

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pGEX ShcA-SH2

Plasmid

#46486

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcA (SHC1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 476-584

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Nck-SH2

Plasmid

#46457

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nck (NCK1 Human)

Use

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 275-377

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-ERBB4 (E317K)

Plasmid

#29528

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed glutamic acid 317 to lysine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only

Showing: 641 - 680 of 1605 results

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