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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,009 results for: D10

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Showing: 321 - 340 of 2009 results

  1. iPEmaxΔR-D10A

    Plasmid
    #239381
    Purpose
    For inverse prime editor using nCas9-D10A-R221K-N394K and M-MLV-RTΔRNase H in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    nCas9(D10A-R221K-N394K), M-MLV-RT(ΔRNase H)
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    D10A, R221K, N394K in Cas9; ΔRNase H in M-MLV RT
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239381-plasmid-map-sequence-id-481782

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  2. iPEmax-D10A

    Plasmid
    #239386
    Purpose
    For inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    nCas9-D10A-R221K-N394K, M-MLV-RT
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    D10A, R221K, N394K in Cas9
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239386-plasmid-map-sequence-id-481780

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  5. pBP-TagR_MCD10

    Plasmid
    #204101
    Purpose
    Type 0 tag-compatible RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD10 medium RBS for assembly with tag part
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204101-plasmid-map-sequence-id-413902

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  6. pBP-R_MCD10

    Plasmid
    #204093
    Purpose
    Type 0 RBS part
    Depositor
    Mikhail Shapiro
    Insert
    MCD10 medium RBS
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204093-plasmid-map-sequence-id-413897

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  13. pEvolvR-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249069
    Purpose
    Expresses nucleus-localized nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249069-plasmid-map-sequence-id-496321

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  14. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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  15. pPD1025 EF1alpha TetON3G in TU6

    Plasmid
    #139262
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV6
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    hEF1alpha
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139262-plasmid-map-sequence-id-325375

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  16. pPD1026 EF1alpha TetON3G in TU2

    Plasmid
    #139258
    Purpose
    Expresses TetON3G under hEF1alpha in TUPV2
    Depositor
    Joshua Leonard
    Insert
    TetON3G
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    hEF1alpha
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139258-plasmid-map-sequence-id-325371

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Showing: 321 - 340 of 2009 results