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pLV x EF1a_EKAREN4-ires-puro

Plasmid

#167828

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

Plasmid

#167819

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4(TA) + PGK-blast

Plasmid

#167827

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

Plasmid

#167820

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4(TA)-ires-puro

Plasmid

#167830

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

Plasmid

#167826

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4(TA)-ires-blast

Plasmid

#167831

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMal-E6LLO

Plasmid

#51281

Purpose

Expresses a fibronectin fusion with listeriolysin targeting EGFR in bacteria

Depositor

Dane Wittrup

Article

Pirie et al Mol Cancer Ther. 2013 Sep;12(9):1774-82.

Insert

E6LLO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMal-E4rGel

Plasmid

#51280

Purpose

Expresses a fibronectin fusion with gelonin targeting EGFR in bacteria

Depositor

Dane Wittrup

Article

Pirie et al J Biol Chem. 2011 Feb 11;286(6):4165-72.

Insert

E4rGel

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

Plasmid

#167832

Purpose

Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

HRAS(N17) (HRAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S17N

Promoter

Ubc, TRE

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-E01DARPin

Plasmid

#198287

Purpose

Produces PVCs retargeted with anti-EGFR DARPin E01

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-E01DARPin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-wt7D12

Plasmid

#125268

Purpose

Expresses wild type 7D12 anti-EGFR nanobodies

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 nanobody

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Trc

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12CTermTAG

Plasmid

#125267

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

CTermTAG

Use

Tags

His-Tag

Expression

Mutation

Promoter

Trc

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Asp73TAG

Plasmid

#125266

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Asp73TAG

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-7D12Gln13TAG

Plasmid

#125264

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

Gln13TAG 7D12

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Gly42TAG

Plasmid

#125265

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Gly42 nanobody

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pX459-dErbB1

Plasmid

#197354

Purpose

A knock-out vector for dog ErbB1.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pKUNOC-ErbB1-mScarlet

Plasmid

#197360

Purpose

Encoding human ErbB1 tagged with mScarlet.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

ERBB1 (EGFR Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-10XHis-GST-HRV-Her2-dL5-Her2

Plasmid

#85624

Purpose

Bacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)

Depositor

Marcel Bruchez

Insert

10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Schistosoma japonicum, Synthetic, Human)

Use

Tags

10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …

Expression

Bacterial

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

T7

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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FLT1-KDR

Plasmid

#86034

Purpose

chimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human VEGFR1-VEGFR2 hybrid (FLT1, KDR Human)

Use

Tags

HA and t-GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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