We narrowed to 389 results for: Tight TRE

1. mIFP12-ZO1-C-14

   Plasmid

   #56265

   Purpose

   Localization: Tight Junctions, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   ZO1 (TJP1 Human)
   

   Use

   Tags

   mIFP12

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. EGFP-uTEV1-MAVS-mTA2

   Plasmid

   #171000

   Purpose

   HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[R537A, R538A]
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[R537A, R538A]

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-uTEV1-MAVS-mTA5

   Plasmid

   #171003

   Purpose

   HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[Y536delinsFIVLI, R537A]

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-uTEV1-MAVS-mTA6

   Plasmid

   #171004

   Purpose

   HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRetroX GFP Mps1 wt

   Plasmid

   #63702

   Purpose

   Inducible expression of GFP-Mps1 fusion in mammalian cells

   Depositor

   Floris Foijer

   Article

   Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   Mps1 (TTK Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX GFP Mps1 DK

   Plasmid

   #63703

   Purpose

   Inducible expression of GFP-Mps1 DK fusion in mammalian cells

   Depositor

   Floris Foijer

   Article

   Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   Mps1 (TTK Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deletion of aa 47-156, kinetochore binding domain

   Promoter

   tight TRE promoter

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCW57-Cx46-IRES-GCaMP6s

   Plasmid

   #188238

   Purpose

   3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

   Insert

   GJA3 (GJA3 Human)
   

   Use

   Lentiviral

   Tags

   GCaMp6s

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AKAP1-EGFP-uTEV1

   Plasmid

   #171005

   Purpose

   HiLITR protease with AKAP1 N-terminal tmd targeting information (Mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   AKAP1(tmd)-EGFP-uTEV1 (AKAP1 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

   Plasmid

   #72924

   Purpose

   Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA Binding Protein 6 (GATA6 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3XAU1 and 3XFLAG

   Expression

   Bacterial

   Mutation

   Serine#37 changed to Alanine;Silent mutations mad…

   Promoter

   TRE tight

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid

    #72617

    Purpose

    Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA Binding Protein 6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Serine#37 changed to Alanine;Silent mutations mad…

    Promoter

    TRE tight

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid

    #137723

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 600-682

    Promoter

    Tight TRE promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PA-mCherry-miniSOG-VE-Cadherin-N-10

    Plasmid

    #57799

    Purpose

    Localization: Tight Junctions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    VE-Cadherin (CDH5 Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

13. AAVS1-iPE-Plus

    Plasmid

    #214062

    Purpose

    inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FUT8-Dox

    Plasmid

    #124631

    Purpose

    Doxycycline-inducible FUT8 expression in mammalian cells

    Depositor

    Ron Weiss

    Article

    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

    Inserts

    Alpha-(1,6)-fucosyltransferase
    Tetracycline repressor A3 mutant
    TagFP635
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight and hEF1a

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. B4GALT1-Dox

    Plasmid

    #124633

    Purpose

    Doxycycline-inducible B4GALT1 expression in mammalian cells

    Depositor

    Ron Weiss

    Article

    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

    Inserts

    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight and hEF1a

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW57-Cx26-IRES-GCaMP6s

    Plasmid

    #188236

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJB2 (GJB2 Human)
    

    Use

    Lentiviral

    Tags

    GCaMP6s

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW57-Cx26G45E-IRES-GCaMP6s

    Plasmid

    #188237

    Purpose

    3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 variant p.G45E and GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

    Insert

    GJB2 (GJB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    p.G45E

    Promoter

    Tight TRE promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid

    #137722

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Truncated at amino acid 1328

    Promoter

    Tight TRE promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRosa26-Acceptor (AG103)

    Plasmid

    #99632

    Purpose

    To insert the Tre-Tight or UAS ifgMosaic Donor vectors

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge