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pDGO186N-KS3

Plasmid

#174303

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSF3-ultraID

Plasmid

#172878

Purpose

expresses ultraID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-S2215Y

Plasmid

#69013

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

S2215Y

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSF3-microID

Plasmid

#172880

Purpose

expresses microID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-V2006I

Plasmid

#69012

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

V2006I

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D][S227D]-FLAG

Plasmid

#199324

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Aspartic Acid

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#172196

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Expression

Yeast

Promoter

10B2 (P1 specific promoter)

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

Plasmid

#199328

Purpose

mutated RHIM motive (interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed V458, Q459,V460 and G461 to alanine

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.0

Plasmid

#176543

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.0
Cas12f-GE ver4.0

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a] mut

Plasmid

#26576

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
Cas12f-GE ver3.0

Plasmid

#176508

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver3.0
Cas12f-GE ver3.0

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag OSM

Plasmid

#12191

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
YFP-MEKK3

Plasmid

#12189

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

MEKK3 (Map3k3 Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-RIPK3[S227D]-FLAG

Plasmid

#199320

Purpose

mutated phosphorylation site (MLKL interaction permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Aspartic Acid

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S227A]-FLAG

Plasmid

#199318

Purpose

mutated phosphorylation site (MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDAG51

Plasmid

#32699

Depositor

Richard C. Austin

Article

Hossain et al J Biol Chem. 2003 Aug 8;278(32):30317-27

Insert

human tdag51 (PHLDA1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
CFP-OSM

Plasmid

#12190

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-RIPK3[S199A][S227A]-FLAG

Plasmid

#199323

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA1-GATA4-opposite orientation

Plasmid

#51928

Purpose

for T7 directed antisense production of mouse GATA4

Depositor

Jeffery Molkentin

Article

Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

Insert

GATA4 reverse orientation (Gata4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
pCDB181

Plasmid

#80692

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHEE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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