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pVAM05

Plasmid

#252056

Purpose

Gentamicin resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Publication

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pMut20_tadDE32B

Plasmid

#247074

Purpose

L-arabinose inducible TadDE-XTEN32-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

TadDE-XTEN16-CasB-UGI

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGH311_wtGFP S65T

Plasmid

#85408

Purpose

lentiviral expression vector for wtGFP S65T

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMut3_pm32B

Plasmid

#247071

Purpose

L-arabinose inducible pmCDA-XTEN32-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

PmCDA-XTEN32-Cas11(CasB)-UGI

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRM200U

Plasmid

#44529

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pYPQ-Cas12j2

Plasmid

#173924

Purpose

Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

NLS-Cas12j2-NLS

Use

CRISPR

Tags

NLS

Expression

Plant

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1594

Plasmid

#11413

Depositor

Sung-Hou Kim

Publication

Wang et al Structure. 2001 Jan 10. 9(1):65-71.

Insert

phosphoserine phosphatase (MJ_1594 M. jannaschii)

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
SM425_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-KAN-DR

Plasmid

#244225

Purpose

Vector for golden gate cloning LbCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQCas12j2

Plasmid

#189780

Purpose

Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

Cas12j2

Use

CRISPR; Gateway compatible cas12j2 entry clone

Tags

NLS

Expression

Plant

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAN29 Phagemid with EG pathway

Plasmid

#253996

Purpose

Phagemid for LySE, carrying a metabolic pathway for ethylene glycol assimilation.

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

EG assimilation pathway

Use

Synthetic Biology

Expression

Bacterial

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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TaRGET-ABE-C3.1 ver4.0

Plasmid

#188250

Purpose

Hypercompact adenine base editors

Depositor

Yong-Sam Kim

Publication

Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

Insert

TaRGET-ABE3.1

Use

CRISPR

Expression

Mammalian

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSJ139 Accessory plasmid with T7 DNAP v9

Plasmid

#254001

Purpose

Accessory plasmid for LySE, carrying error-prone T7 DNAP-TadDE (v9) for optimised mutational spectrum.

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

T7 DNAP v9

Expression

Bacterial

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-Pls4-sfGFP

Plasmid

#205751

Purpose

Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.

Depositor

Jerome Bonnet

Publication

Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

Type

Empty backbone

Use

Shuttle vector gram+ gram-

Expression

Bacterial

Promoter

Pls4 (Phyperspank mutant, IPTG inducible)

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-IRF2

Plasmid

#232642

Purpose

transient expresses IRF2 in mammalian cells

Depositor

Chen-Yang Shen

Publication

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

IRF2 (IRF2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pZG61

Plasmid

#202575

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-TCF7L2

Plasmid

#232644

Purpose

transient expresses TCF7L2 in mammalian cells

Depositor

Chen-Yang Shen

Publication

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

TCF7L2 (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-GATA4

Plasmid

#232647

Purpose

transient expresses GATA4 in mammalian cells

Depositor

Chen-Yang Shen

Publication

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

GATA4 (GATA4 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pACT-HAB1

Plasmid

#241276

Purpose

Yeast two hybrid vector expressing AD-HAB1

Depositor

Sean Cutler

Publication

Park et al Science. 2009 May 22;324(5930):1068-71.

Insert

HAB1 (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE1_2

Plasmid

#160764

Purpose

Suppress POLE1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_2

Use

Lentiviral

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1P REV3L_1

Plasmid

#160797

Purpose

Suppress REV3L

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shREV3L_1

Use

Lentiviral

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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