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Showing: 321 - 340 of 446 results

  5. pTon1(il11)

    Plasmid
    #233647
    Purpose
    Expression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Inserts
    interleukin-11.L (350-913) (il11.L Frog)
    target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Tags
    Expression
    Mutation
    Some synonymous substitutions were introduced in…
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233647-plasmid-map-sequence-id-468199

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  6. pCW-Cdt1ΔCy-mCherry-Puro

    Plasmid
    #193764
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    Cdt1ΔCy-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193764-plasmid-map-sequence-id-380225

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  7. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

    Plasmid
    #229795
    Purpose
    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    TRE and CAG
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229795-plasmid-map-sequence-id-461775

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  11. TRE3G-PE2-P2A-BFP

    Plasmid
    #231580
    Purpose
    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein
    Depositor
    Jesse Engreitz
    Article
    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.
    Insert
    PE2-P2A-BFP
    Use
    Lentiviral
    Tags
    P2A-BFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231580-plasmid-map-sequence-id-462114

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  12. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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  13. pCW-ND-Cdt1-mCherry-Puro

    Plasmid
    #193766
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193766-plasmid-map-sequence-id-380221

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  14. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

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  15. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100596-plasmid-map-sequence-id-207339

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  17. pCW-ND-Cdt1-HA-Puro

    Plasmid
    #193767
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-HA (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193767-plasmid-map-sequence-id-380216

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  18. PB-TRE3G-dN40-TP53-CCRB

    Plasmid
    #236774
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven short-form of TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-40
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    236774-plasmid-map-sequence-id-473566

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  19. PB-TRE3G-TP53-CCRB

    Plasmid
    #236773
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven full-length TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    236773-plasmid-map-sequence-id-473536

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  20. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

    Plasmid
    #163701
    Purpose
    Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Article
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Inserts
    SOX2-3xHA-P2A-tagBFP (SOX2 Human)
    rtTA
    T2-sgRNA
    Use
    AAV and CRISPR
    Tags
    3x-HA and P2A-tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and TRE3G
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163701-plasmid-map-sequence-id-325288

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Showing: 321 - 340 of 446 results